Recombinant humaan okt-3/4 eiwit

POu domain, class 5, transcription factor 1, also known as OCT4, binds to the octamer motif (5'-ATTTGCAT-3') . This protein forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, uTF1 and ZFP206. It is critical for early embryogenesis and for embryonic stem cell pluripotency. Recombinant human OCT4 protein, fused to His-tag at N-terminus, was expressed in E. coli.

POU domain, class 5, transcription factor 1 isoform 3, Oct-3, Oct-4, OCT3, OCT4, OTF-3, OTF3, OTF4

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

32.2 kDa (285aa)

38263, POU domain, class 5, transcription factor 1 isoform 3, Oct-3, Oct-4, OCT3, OCT4, OTF-3, OTF3, OTF4, ATGP1948-10 µg, ATGP1948-20 µg, ATGP1948-50 µg, ATGP1948-100 µg, ATGP1948-250 µg, ATGP1948-500 µg, ATGP1948-1 mg, ATGP1948-10, ATGP1948-20, ATGP1948-50, ATGP1948-100, ATGP1948-250, ATGP1948-500, ATGP1948-1

Takahashi K., et al. (2007) Cell. 131:861-872; ; Lee J., et al. (2008) Int. J. Biochem. Cell Biol. 40:1043-1054

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Development

NP_001272916

Q01860

Human

MGSSHHHHHH SSGLVPRGSH MHFYRLFLGA TRRFLNPEWK GEIDNWCVYV LTSLLPFKIQ SQDIKALQKE LEQFAKLLKQ KRITLGYTQA DVGLTLGVLF GKVFSQTTIC RFEALQLSFK NMCKLRPLLQ KWVEEADNNE NLQEICKAET LVQARKRKRT SIENRVRGNL ENLFLQCPKP TLQQISHIAQ QLGLEKDVVR VWFCNRRQKG KRSSSDYAQR EDFEAAGSPF SGGPVSFPLA PGPHFGTPGY GSPHFTALYS SVPFPEGEAF PPVSVTTLGS PMHSN