Recombinant humaan OLA1-eiwit

OLA1, also known as obg-like ATPase 1, functions as a negative regulator of the cellular antioxidant response independent of transcriptional processes. OLA1 suppresses the antioxidant response through nontranscriptional mechanisms. Recombinant human OLA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Obg-like ATPase 1, DOC45, GBP45, GTBP9, GTPBP9, PTD004

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

47.3 kDa (420aa) confirmed by MALDI-TOF

OLA1, Obg-like ATPase 1, DOC45, GBP45, GTBP9, GTPBP9, PTD004, ATGP1660-10 µg, ATGP1660-20 µg, ATGP1660-50 µg, ATGP1660-100 µg, ATGP1660-250 µg, ATGP1660-500 µg, ATGP1660-1 mg, ATGP1660-10, ATGP1660-20, ATGP1660-50, ATGP1660-100, ATGP1660-250, ATGP1660-500, ATGP1660-1

Choudhary C., et al. (2009) Science. 325:834-840; ; Koller-Eichhorn R., et al. (2007) J. Biol. Chem. 282:19928-19937

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl

Enzymes & Proteases

NP_037473

Q9NTK5

Human

MGSSHHHHHH SSGLVPRGSH MGSHMPPKKG GDGIKPPPII GRFGTSLKIG IVGLPNVGKS TFFNVLTNSQ ASAENFPFCT IDPNESRVPV PDERFDFLCQ YHKPASKIPA FLNVVDIAGL VKGAHNGQGL GNAFLSHISA CDGIFHLTRA FEDDDITHVE GSVDPIRDIE IIHEELQLKD EEMIGPIIDK LEKVAVRGGD KKLKPEYDIM CKVKSWVIDQ KKPVRFYHDW NDKEIEVLNK HLFLTSKPMV YLVNLSEKDY IRKKNKWLIK IKEWVDKYDP GALVIPFSGA LELKLQELSA EERQKYLEAN MTQSALPKII KAGFAALQLE YFFTAGPDEV RAWTIRKGTK APQAAGKIHT DFEKGFIMAE VMKYEDFKEE GSENAVKAAG KYRQQGRNYI VEDGDIIFFK FNTPQQPKKK