Recombinant humaan ORC6-eiwit

Origin recognition complex subunit 6, also known as ORC6, belongs to the origin recognition complex (ORC) . ORC is a highly conserved heterohexameric protein complex that associates with DNA at or near sites of initiation of DNA replication. All six ORC subunits are essential for initiation of DNA replication, and ORC may be involved in regulation of gene expression in response to stress. ORC6 is also expressed constantly throughout the cell cycle. ORC2, ORC3, ORC4 and ORC5 form a core complex upon which ORC6 and ORC1 assemble. Recombinant human ORC6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Origin recognition complex subunit 6, ORC6L

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

30.5 kDa (275aa) confirmed by MALDI-TOF

ORC6, Origin recognition complex subunit 6, ORC6L, ATGP2351-10 µg, ATGP2351-20 µg, ATGP2351-50 µg, ATGP2351-100 µg, ATGP2351-250 µg, ATGP2351-500 µg, ATGP2351-1 mg, ATGP2351-10, ATGP2351-20, ATGP2351-50, ATGP2351-100, ATGP2351-250, ATGP2351-500, ATGP2351-1

Kreitz S., et al. (2000) J Biol Chem. 276:6337-6342; ; Mendez J., et al. (2000) Mol Cell Biol. 20:8602-8612.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 2mM DTT

Epigenomics (Transcription & Translation)

NP_055136

Q9Y5N6

Human

MGSSHHHHHH SSGLVPRGSH MGSMGSELIG RLAPRLGLAE PDMLRKAEEY LRLSRVKCVG LSARTTETSS AVMCLDLAAS WMKCPLDRAY LIKLSGLNKE TYQSCLKSFE CLLGLNSNIG IRDLAVQFSC IEAVNMASKI LKSYESSLPQ TQQVDLDLSR PLFTSAALLS ACKILKLKVD KNKMVATSGV KKAIFDRLCK QLEKIGQQVD REPGDVATPP RKRKKIVVEA PAKEMEKVEE MPHKPQKDED LTQDYEEWKR KILENAASAQ KATAE