Recombinant humaan Ornithine Carbamoyltransferase/OTC-eiwit

OTC, also called ornithine carbamoyltransferase, belongs to the ATCase/OTCase family. OTC plays a vital role in the urea cycle, catalyzing the second step in this pathway: the formation of L-citrulline from L-orthinine and carbamoyl phosphate. In humans, the urea cycle is an important pathway to detoxification of ammonia. Mutations in the gene encoding OTC are associated with the X-linked disorder OTCD (ornithine carbamoyltransferase deficiency) . OTCD is a disorder of the urea cycle characterized by hyperammonemia. Recombinant human OTC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ornithine carbamoyltransferase, OCTD, OTCase

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

38.9 kDa (347aa) confirmed by MALDI-TOF

Ornithine Carbamoyltransferase, OTC, ornithine carbamoyltransferase, OCTD, OTCase, ATGP1473-10 µg, ATGP1473-20 µg, ATGP1473-50 µg, ATGP1473-100 µg, ATGP1473-250 µg, ATGP1473-500 µg, ATGP1473-1 mg, ATGP1473-10, ATGP1473-20, ATGP1473-50, ATGP1473-100, ATGP1473-250, ATGP1473-500, ATGP1473-1

Trivedi, M., et al. (2001) J. Clin. Gastroenterol. 32: 340-343.; ; Yamaguchi, S., et al. (2006) Hum. Mutat. 27: 626-632.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM MES buffer (pH 6.0) containing 2mM DTT, 10% glycerol, 100 mM NaCl

Metabolism

NP_000522.3

P00480

Human

NKVQL KGRDLLTLKN FTGEEIKYML WLSADLKFRI KQKGEYLPLL QGKSLGMIFE KRSTRTRLST ETGFALLGGH PCFLTTQDIH LGVNESLTDT ARVLSSMADA VLARVYKQSD LDTLAKEASI PIINGLSDLY HPIQILADYL TLQEHYSSLK GLTLSWIGDG NNILHSIMMS AAKFGMHLQA ATPKGYEPDA SVTKLAEQYA KENGTKLLLT NDPLEAAHGG NVLITDTWIS MGQEEEKKKR LQAFQGYQVT MKTAKVAASD WTFLHCLPRK PEEVDDEVFY SPRSLVFPEA ENRKWTIMAV MVSLLTDYSP QLQKPKF