Recombinant humaan OSGEP-eiwit

OSGEP belongs to the KAE1 / YgjD family. This protein is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Recombinant human OSGEP protein, fused to His-tag at N-terminus, was expressed in E. coli.

Probable tRNA threonylcarbamoyladenosine biosynthesis protein OSGEP, GCPL1, KAE1, OSGEP1, PRSMG1

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.5 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

38.8 kDa (358aa)

OSGEP, probable tRNA threonylcarbamoyladenosine biosynthesis protein OSGEP, GCPL1, KAE1, OSGEP1, PRSMG1, ATGP2793-10 µg, ATGP2793-20 µg, ATGP2793-50 µg, ATGP2793-100 µg, ATGP2793-250 µg, ATGP2793-500 µg, ATGP2793-1 mg, ATGP2793-10, ATGP2793-20, ATGP2793-50, ATGP2793-100, ATGP2793-250, ATGP2793-500, ATGP2793-1

Seki Y., et al (2002) . Gene 285:101-108; ; Burkard T.R., et al (2011) . BMC Syst. Biol. 5:17-17

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea

Epigenomics (Transcription & Translation)

NP_060277

Q9NPF4

Human

MGSSHHHHHH SSGLVPRGSH MGSMPAVLGF EGSANKIGVG VVRDGKVLAN PRRTYVTPPG TGFLPGDTAR HHRAVILDLL QEALTESGLT SQDIDCIAYT KGPGMGAPLV SVAVVARTVA QLWNKPLVGV NHCIGHIEMG RLITGATSPT VLYVSGGNTQ VIAYSEHRYR IFGETIDIAV GNCLDRFARV LKISNDPSPG YNIEQMAKRG KKLVELPYTV KGMDVSFSGI LSFIEDVAHR MLATGECTPE DLCFSLQETV FAMLVEITER AMAHCGSQEA LIVGGVGCNV RLQEMMATMC QERGARLFAT DERFCIDNGA MIAQAGWEMF RAGHRTPLSD SGVTQRYRTD EVEVTWRD