Recombinant humaan Osteoactivine/GPNMB-eiwit

GPNMB is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. The protein shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. Recombinant human GPNMB protein, fused to His-tag at N-terminus, was expressed in E. coli.

Transmembrane glycoprotein NMB, Glycoprotein NMB, Glycoprotein nonmetastatic melanoma protein B, Hematopoietic growth factor inducible neurokinin-1, HGFIN

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

53.2 kDa (476aa)

GPNMB, Glycoprotein (transmembrane) nmb, HGFIN, NMB, ATGP2280-10 µg, ATGP2280-20 µg, ATGP2280-50 µg, ATGP2280-100 µg, ATGP2280-250 µg, ATGP2280-500 µg, ATGP2280-1 mg, ATGP2280-10, ATGP2280-20, ATGP2280-50, ATGP2280-100, ATGP2280-250, ATGP2280-500, ATGP2280-1

Rose A.A., et al. (2010) PLoS ONE. 5:e12093-e12093; ; Patel-Chamberlin M., et al. (2011) Kidney Int. 79:1138-1148

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Cancer

NP_002501

Q14956

Human

MGSSHHHHHH SSGLVPRGSH MGSAKRFHDV LGNERPSAYM REHNQLNGWS SDENDWNEKL YPVWKRGDMR WKNSWKGGRV QAVLTSDSPA LVGSNITFAV NLIFPRCQKE DANGNIVYEK NCRNEAGLSA DPYVYNWTAW SEDSDGENGT GQSHHNVFPD GKPFPHHPGW RRWNFIYVFH TLGQYFQKLG RCSVRVSVNT ANVTLGPQLM EVTVYRRHGR AYVPIAQVKD VYVVTDQIPV FVTMFQKNDR NSSDETFLKD LPIMFDVLIH DPSHFLNYST INYKWSFGDN TGLFVSTNHT VNHTYVLNGT FSLNLTVKAA APGPCPPPPP PPRPSKPTPS LGPAGDNPLE LSRIPDENCQ INRYGHFQAT ITIVEGILEV NIIQMTDVLM PVPWPESSLI DFVVTCQGSI PTEVCTIISD PTCEITQNTV CSPVDVDEMC LLTVRRTFNG SGTYCVNLTL GDDTSLALTS TLISVP