Recombinant humaan Osteopontine/OPN-eiwit

Osteopontin (OPN), also known as Secreted phosphoprotein 1 (SPP1), is a human gene product which is conserved in other species. This protein is glycoprotein that was first identified in osteoblasts and plays an important role in bone remodeling, immune functions in fibroblasts, macrophages, and lymphocytes during inflammation and wound healing. Recombinant human Osteopontin protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Human OPN stimulates cell adhesion of the HEK293 human embryonic kidney cells. This effect is more to 40%.

OPN, SPP-1, BNSP, BSPI, ETA-1, Bone sialoprotein 1, BSP I.Early T lymphocyte activation 1, ETA 1, ETA1, MGC110940, Nephropontin, Secreted phosphoprotein 1, SPP 1, SPP1, urinary stone protein, uropontin

E.coli

Human

His-Tag

SDS-PAGE, Bioactivity

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When cells are added to OPN coated plates 10 µg/mL. This effect is more to 40%.

36.2 kDa (321aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Osteopontin, OPN, OPN, SPP-1, BNSP, BSPI, ETA-1, Bone sialoprotein 1, BSP I.Early T lymphocyte activation 1, ETA 1, ETA1, MGC110940, Nephropontin, Secreted phosphoprotein 1, SPP 1, SPP1, urinary stone protein, uropontin, ATGP3951-10 µg, ATGP3951-20 µg, ATGP3951-50 µg, ATGP3951-100 µg, ATGP3951-250 µg, ATGP3951-500 µg, ATGP3951-1 mg, ATGP3951-10, ATGP3951-20, ATGP3951-50, ATGP3951-100, ATGP3951-250, ATGP3951-500, ATGP3951-1, ATGP0274

Koh A., et al. (2007) . Immunology. 122 (4) :466-75; ; Sodek J., et al. (2006) . J Dent Res. 85 (5) :404-15;

Jung SH, et al. Integrative proteomic profiling of protein activity and interactions using protein arrays. (Mol Cell Proteomics. 2012) {https://pubmed.ncbi.nlm.nih.gov/22843993/}; ; Jung SH, et al. Identification of transglutaminase 2 kinase substrates using a novel on-chip activity assay. (Biosens Bioelectron. 2016) {https://pubmed.ncbi.nlm.nih.gov/27040940/}; ; Jung SH, et al. Systematic investigation of protein kinase A substrate proteins using on-chip protein kinase kinetic profiling. (Analyst. 2017) {https://pubmed.ncbi.nlm.nih.gov/28536714/}; ; Kim ID, et al. Osteopontin Peptide Icosamer Containing RGD and SLAYGLR Motifs Enhances the Motility and Phagocytic Activity of Microglia. (Exp Neurobiol. 2017) {https://pubmed.ncbi.nlm.nih.gov/29302201/}; ; Jung SH, et al. On-chip dual enzyme activity assay to investigate regulation of the transamidase and kinase activities of transglutaminase 2. (Anal Chim Acta. 2018) {https://pubmed.ncbi.nlm.nih.gov/29866275/}; ; Jung SH, et al. Array-based Investigation of Amino Acids Responsible for Regulation of Transamidase and Kinase Activities of Transglutaminase 2. (BioChip J. 2019) {https://doi.org/10.1007/s13206-019-3307-3}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 10% glycerol, 2mM EDTA

Hormones & Cytokines

NP_001035147

P10451

Human

RSMIPVKQAD SGSSEEKQLY NKYPDAVATW LNPDPSQKQN LLAPQNAVSS EETNDFKQET LPSKSNESHD HMDDMDDEDD DDHVDSQDSI DSNDSDDVDD TDDSHQSDES HHSDESDELV TDFPTDLPAT EVFTPVVPTV DTYDGRGDSV VYGLRSKSKK FRRPDIQYPD ATDEDITSHM ESEELNGAYK AIPVAQDLNA PSDWDSRGKD SYETSQLDDQ SAETHSHKQS RLYKRKANDE SNEHSDVIDS QELSKVSREF HSHEFHSHED MLVVDPKSKE EDKHLKFRIS HELDSASSEV N