Recombinant humaan Otubain-2/OTUB2-eiwit

OTuB2 (ubiquitin thioesterase OTuB2) belongs to the peptidase C65 family. OTuB2 functions as a hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Recombinant human OTuB2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ubiquitin thioesterase OTuB2, OTB2, OTu2, TimntanOTUB2 aUgitin thioesterase 2

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

29.3 kDa (254aa) confirmed by MALDI-TOF

Otubain2, ubiquitin thioesterase OTuB2, OTB2, OTu2, ATGP1038-10 µg, ATGP1038-20 µg, ATGP1038-50 µg, ATGP1038-100 µg, ATGP1038-250 µg, ATGP1038-500 µg, ATGP1038-1 mg, ATGP1038-10, ATGP1038-20, ATGP1038-50, ATGP1038-100, ATGP1038-250, ATGP1038-500, ATGP1038-1

Balakirev MY, et al. (2003) EMBO Rep 4 (5) : 517-22.; ; Edelmann M.J., et al. (2009) Biochem. J. 418:379-390

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50 mM NaCl

Ubiquitination

NP_075601

Q96DC9

Human

MSETSFNLIS EKCDILSILR DHPENRIYRR KIEELSKRFT AIRKTKGDGN CFYRALGYSY LESLLGKSRE IFKFKERVLQ TPNDLLAAGF EEHKFRNFFN AFYSVVELVE KDGSVSSLLK VFNDQSASDH IVQFLRLLTS AFIRNRADFF RHFIDEEMDI KDFCTHEVEP MATECDHIQI TALSQALSIA LQVEYVDEMD TALNHHVFPE AATPSVYLLY KTSHYNILYA ADKH