Recombinant humaan Otubaïne-1/OTUB1-eiwit

Otubain 1, also known as OTuB1, is a member of the OuT (ovarian tumor) superfamily of predicted cysteine proteases and inhibits cytokine gene transcription in the immune system via its interaction with a ubiquitin protease and E3 ubiquitin ligase. This protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function of polyubiquitin chain growth. Recombinant OTuB1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Ubiquitin thioesterase Otubain 1, OTB1, OTu1, ubiquitin thioesterase Otubain 1 Deubiquitinating enzyme OTuB1, OTu domain containing ubiquitin aldehyde binding protein 1, Otubain 1, ubiquitin specific processing protease OTuB1, ubiquitin thioesterase OTuB1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

33.4 kDa (291aa) confirmed by MALDI-TOF

Otubain1, ubiquitin thioesterase Otubain 1, OTB1, OTu1, ubiquitin thioesterase Otubain 1 Deubiquitinating enzyme OTuB1, OTu domain containing ubiquitin aldehyde binding protein 1, Otubain 1, ubiquitin specific processing protease OTuB1, ubiquitin thioesterase OTuB1., ATGP0354-10 µg, ATGP0354-20 µg, ATGP0354-50 µg, ATGP0354-100 µg, ATGP0354-250 µg, ATGP0354-500 µg, ATGP0354-1 mg, ATGP0354-10, ATGP0354-20, ATGP0354-50, ATGP0354-100, ATGP0354-250, ATGP0354-500, ATGP0354-1

Balakirev MY., et al. (2003) EMBO Rep. 4 (5) :517-22. ; ; Soares L., et al. (2004) Nat Immunol. 5 (1) :45-54.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Enzymes & Proteases

NP_060140

Q96FW1

Human

MAAEEPQQQK QEPLGSDSEG VNCLAYDEAI MAQQDRIQQE IAVQNPLVSE RLELSVLYKE YAEDDNIYQQ KIKDLHKKYS YIRKTRPDGN CFYRAFGFSH LEALLDDSKE LQRFKAVSAK SKEDLVSQGF TEFTIEDFHN TFMDLIEQVE KQTSVADLLA SFNDQSTSDY LVVYLRLLTS GYLQRESKFF EHFIEGGRTV KEFCQQEVEP MCKESDHIHI IALAQALSVS IQVEYMDRGE GGTTNPHIFP EGSEPKVYLL YRPGHYDILY K