Recombinant humaan OXSM-eiwit

OXSM is a beta-ketoacyl synthetase. The enzyme is required for elongation of fatty acid chains in the mitochondria. Alternatively spliced transcript variants have been described. Recombinant human OXSM protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

3-oxoacyl-ACP synthase mitochondrial, 3-oxoacyl-ACP synthase, mitochondrial, FASN2D, KASI, KS

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

48.1 kDa (455aa) confirmed by MALDI-TOF

OXSM, 3-oxoacyl-ACP synthase mitochondrial, 3-oxoacyl-ACP synthase, mitochondrial, FASN2D, KASI, KS, ATGP2347-10 µg, ATGP2347-20 µg, ATGP2347-50 µg, ATGP2347-100 µg, ATGP2347-250 µg, ATGP2347-500 µg, ATGP2347-1 mg, ATGP2347-10, ATGP2347-20, ATGP2347-50, ATGP2347-100, ATGP2347-250, ATGP2347-500, ATGP2347-1

Zhang L., et al (2005) . J. Biol. Chem. 280:12422-12429 (2005)

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Metabolism

NP_060367

Q9NWU1

Human

MGSSHHHHHH SSGLVPRGSH MGSKRKFFGT VPISRLHRRV VITGIGLVTP LGVGTHLVWD RLIGGESGIV SLVGEEYKSI PCSVAAYVPR GSDEGQFNEQ NFVSKSDIKS MSSPTIMAIG AAELAMKDSG WHPQSEADQV ATGVAIGMGM IPLEVVSETA LNFQTKGYNK VSPFFVPKIL VNMAAGQVSI RYKLKGPNHA VSTACTTGAH AVGDSFRFIA HGDADVMVAG GTDSCISPLS LAGFSRARAL STNSDPKLAC RPFHPKRDGF VMGEGAAVLV LEEYEHAVQR RARIYAEVLG YGLSGDAGHI TAPDPEGEGA LRCMAAALKD AGVQPEEISY INAHATSTPL GDAAENKAIK HLFKDHAYAL AVSSTKGATG HLLGAAGAVE AAFTTLACYY QKLPPTLNLD CSEPEFDLNY VPLKAQEWKT EKRFIGLTNS FGFGGTNATL CIAGL