Recombinant humaan p18INK4c/CDKN2C-eiwit

CDKN2C (Cyclin-dependent kinase 4 inhibitor C) belongs to the CDKN2 cyclin-dependent kinase inhibitor family. It has been shown to interact with CDK4 or CDK6, and prevent the activation of the CDK kinases, thus function as a cell growth regulator that controls cell cycle G1 progression. And this protein suppresses the cell growth and proliferation with a correlated dependence on endogenous retinoblastoma protein RB. Studies in the knockout mice suggested the roles of this gene in regulating spermatogenesis, as well as in suppressing tumorigenesis. Recombinant human CDKN2C protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Cyclin-dependent kinase 4 inhibitor C, INK4C, p18, p18-INK4C

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

20.7 kDa (192aa) confirmed by MALDI-TOF

P18INK4CDKN2C, Cyclin-dependent kinase 4 inhibitor C, INK4C, p18, p18-INK4C, ATGP1377-10 µg, ATGP1377-20 µg, ATGP1377-50 µg, ATGP1377-100 µg, ATGP1377-250 µg, ATGP1377-500 µg, ATGP1377-1 mg, ATGP1377-10, ATGP1377-20, ATGP1377-50, ATGP1377-100, ATGP1377-250, ATGP1377-500, ATGP1377-1

Blais A, et al. (1998) .) Biochem Biophys Res Commun 247 (1) : 146-53.; ; Serrano M, et al. (1994) . Nature 366 (6456) : 704-7.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 200 mM NaCl

Cancer

NP_001253

P42773

Human

MGSSHHHHHH SSGLVPRGSH MGSHMAEPWG NELASAAARG DLEQLTSLLQ NNVNVNAQNG FGRTALQVMK LGNPEIARRL LLRGANPDLK DRTGFAVIHD AARAGFLDTL QTLLEFQADV NIEDNEGNLP LHLAAKEGHL RVVEFLVKHT ASNVGHRNHK GDTACDLARL YGRNEVVSLM QANGAGGATN LQ