Recombinant humaan p21/CIP1/CDKN1A-eiwit

CDKN1A, also known as P21 or WAF1, plays a critical role in the cellular response to DNA damage, and its overexpression results in cell cycle arrest. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S. However, CDKN1A may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D. This protein may be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Recombinant human CDKN1A protein, fused to His-tag at N-terminus, was expressed in E. coli.

Cyclin-dependent kinase inhibitor 1A, P21, CIP1, SDI1, WAF1, CAP20, CDKN1, MDA-6, p21CIP1

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

20.2 kDa (184aa)

P21, CIP1, CDKN1A, Cyclin-dependent kinase inhibitor 1A, P21, CIP1, SDI1, WAF1, CAP20, CDKN1, MDA-6, p21CIP1, ATGP1725-10 µg, ATGP1725-20 µg, ATGP1725-50 µg, ATGP1725-100 µg, ATGP1725-250 µg, ATGP1725-500 µg, ATGP1725-1 mg, ATGP1725-10, ATGP1725-20, ATGP1725-50, ATGP1725-100, ATGP1725-250, ATGP1725-500, ATGP1725-1

EI-Deiry W S., et al. (1994) Cancer Res. 54:1169-1174.; ; Harper J W., et al. (1993) Cell. 75:805-816.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2M urea, 10% glycerol, 0.1M NaCl

Cancer

NP_000380.1

P38936

Human

MSEPAGDVRQ NPCGSKACRR LFGPVDSEQL SRDCDALMAG CIQEARERWN FDFVTETPLE GDFAWERVRG LGLPKLYLPT GPRRGRDELG GGRRPGTSPA LLQGTAEEDH VDLSLSCTLV PRSGEQAEGS PGGPGDSQGR KRRQTSMTDF YHSKRRLIFS KRKP