Recombinant humaan p38-alfa/MAPK14-eiwit

MAPK14 (Mitogen-activated protein kinase 14) is a member of the MAP kinase family. MAPK14 is most closely related to p38 MAP kinases (MAPKs) . MAPKs are activated primarily in response to inflammatory cytokines and cellular stress, and inhibitors which target the MAPK14 and MAPK11 have shown potential for the treatment of inflammatory disease. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress-related transcription and cell cycle regulation, as well as in genotoxic stress response. Recombinant human MAPK14 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Mitogen-activated protein kinase 14, CSBP, CSBP1, CSBP2, CSPB1, EXIP, Mxi2, p38, p38ALPHA, PRKM14, PRKM15, RK, SAPK2A

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

43.7 kDa (383aa) confirmed by MALDI-TOF

P38 a, MAPK14, Mitogen-activated protein kinase 14, CSBP, CSBP1, CSBP2, CSPB1, EXIP, Mxi2, p38, p38ALPHA, PRKM14, PRKM15, RK, SAPK2A, ATGP1300-10 µg, ATGP1300-20 µg, ATGP1300-50 µg, ATGP1300-100 µg, ATGP1300-250 µg, ATGP1300-500 µg, ATGP1300-1 mg, ATGP1300-10, ATGP1300-20, ATGP1300-50, ATGP1300-100, ATGP1300-250, ATGP1300-500, ATGP1300-1

Beardmore VA, et al. (2005) Mol Cell Biol. 25 (23) :10454-64.; ; Chen, Z, et al (2001) J. Biol. Chem. (united States) . 276 (19) : 16070-5

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100 mM NaCl

Signal Transduction

NP_620581.1

Q16539

Human

MSQERPT FYRQELNKTI WEVPERYQNL SPVGSGAYGS VCAAFDTKTG LRVAVKKLSR PFQSIIHAKR TYRELRLLKH MKHENVIGLL DVFTPARSLE EFNDVYLVTH LMGADLNNIV KCQKLTDDHV QFLIYQILRG LKYIHSADII HRDLKPSNLA VNEDCELKIL DFGLARHTDD EMTGYVATRW YRAPEIMLNW MHYNQTVDIW SVGCIMAELL TGRTLFPGTD HIDQLKLILR LVGTPGAELL KKISSESARN YIQSLTQMPK MNFANVFIGA NPLAVDLLEK MLVLDSDKRI TAAQALAHAY FAQYHDPDDE PVADPYDQSF ESRDLLIDEW KSLTYDEVIS FVPPPLDQEE MES