Recombinant humaan p38 bèta/MAPK11-eiwit

MAPK11 (Mitogen-activated protein kinase 11) is a member of the MAP kinase family. This kinase is most closely related to p38 MAP kinases (MAPKs) . MAPKs are activated primarily in response to inflammatory cytokines and cellular stress, and inhibitors which target the MAPK14 and MAPK11 have shown potential for the treatment of inflammatory disease. MAPK11 has been shown to interact with HDAC3 and Promyelocytic leukemia protein. This kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment, by cytokines, or by environmental stress. Recombinant human MAPK11 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Mitogen-activated protein kinase 11, p38-2, P38B, p38Beta, P38BETA2, PRKM11, SAPK2, SAPK2B

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

43.7 kDa (387aa) confirmed by MALDI-TOF

P38 b, MAPK11, Mitogen-activated protein kinase 11, p38-2, P38B, p38Beta, P38BETA2, PRKM11, SAPK2, SAPK2B, ATGP1299-10 µg, ATGP1299-20 µg, ATGP1299-50 µg, ATGP1299-100 µg, ATGP1299-250 µg, ATGP1299-500 µg, ATGP1299-1 mg, ATGP1299-10, ATGP1299-20, ATGP1299-50, ATGP1299-100, ATGP1299-250, ATGP1299-500, ATGP1299-1

Beardmore VA, et al. (2005) Mol Cell Biol. 25 (23) :10454-64.; ; Mahlknecht u, et al. (2004) J Immunol. 173 (6) :3979-90.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 100 mM NaCl

Signal Transduction

NP_002742.3

Q15759

Human

MSGPRAG FYRQELNKTV WEVPQRLQGL RPVGSGAYGS VCSAYDARLR QKVAVKKLSR PFQSLIHARR TYRELRLLKH LKHENVIGLL DVFTPATSIE DFSEVYLVTT LMGADLNNIV KCQALSDEHV QFLVYQLLRG LKYIHSAGII HRDLKPSNVA VNEDCELRIL DFGLARQADE EMTGYVATRW YRAPEIMLNW MHYNQTVDIW SVGCIMAELL QGKALFPGSD YIDQLKRIME VVGTPSPEVL AKISSEHART YIQSLPPMPQ KDLSSIFRGA NPLAIDLLGR MLVLDSDQRV SAAEALAHAY FSQYHDPEDE PEAEPYDESV EAKERTLEEW KELTYQEVLS FKPPEPPKPP GSLEIEQ