Recombinant humaan p38 gamma/MAPK12 eiwit

Mitogen-activated protein kinase 12, also known as MAPK12, belongs to the MAP kinase family. MAPK12 functions as a signal transducer during differentiation of myoblasts to myotubes. Expressed in different areas throughout the body with common expression patterns in heart, p38 proteins use magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins. Recombinant human MAPK12 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Mitogen-activated protein kinase 12, ERK3, ERK6, P38GAMMA, PRKM12, SAPK-3, SAPK3

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

44.1 kDa (387aa) confirmed by MALDI-TOF

P38 g, MAPK12, Mitogen-activated protein kinase 12, ERK3, ERK6, P38GAMMA, PRKM12, SAPK-3, SAPK3, ATGP1313-10 µg, ATGP1313-20 µg, ATGP1313-50 µg, ATGP1313-100 µg, ATGP1313-250 µg, ATGP1313-500 µg, ATGP1313-1 mg, ATGP1313-10, ATGP1313-20, ATGP1313-50, ATGP1313-100, ATGP1313-250, ATGP1313-500, ATGP1313-1

Han J., et al. (1995) Biochim Biophys Acta. 1265:224-227.; ; Court N W., et al. (2002) J Mol Cell Cardiol. 34:413-426.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Signal Transduction

NP_002960

P53778

Human

MSSPPPARSG FYRQEVTKTA WEVRAVYRDL QPVGSGAYGA VCSAVDGRTG AKVAIKKLYR PFQSELFAKR AYRELRLLKH MRHENVIGLL DVFTPDETLD DFTDFYLVMP FMGTDLGKLM KHEKLGEDRI QFLVYQMLKG LRYIHAAGII HRDLKPGNLA VNEDCELKIL DFGLARQADS EMTGYVVTRW YRAPEVILNW MRYTQTVDIW SVGCIMAEMI TGKTLFKGSD HLDQLKEIMK VTGTPPAEFV QRLQSDEAKN YMKGLPELEK KDFASILTNA SPLAVNLLEK MLVLDAEQRV TAGEALAHPY FESLHDTEDE PQVQKYDDSF DDVDRTLDEW KRVTYKEVLS FKPPRQLGAR VSKETPL