Recombinant humaan p47phox/NCF1-eiwit

Neutrophil cytosolic factor 1, also known as NCF1, is a cytosolic subunit of neutrophil NADPH oxidase. NCF1, along with NCF2 and a membrane bound cytochrome b558, is required for activation of the latent NADPH oxidase necessary for superoxide production. Defects in NCF1, often resulting from recombination between NCF1 and a nearby homologous pseudogene, cause chronic granulomatous disease. Recombinant human NCF1 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Neutrophil cytosol factor 1, 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-47K, Neutrophil NADPH oxidase factor 1, Nox organizer 2, Nox-organizing protein 2, SH3 and PX domain-containing protein 1A, NADPH oxidase organizer 2, NOXO2, SH3PXD1A, NCF1A

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

45.7 kDa (398aa) confirmed by MALDI-TOF

SH3PXD1A, SH3 and PX domain-containing protein 1A, SH3 and PX domain containing protein 1A, p47phox, Nox-organizing protein 2, NOXO2, Nox organizing protein2, Nox organizing protein 2, Nox organizer 2, Neutrophil NADPH oxidase factor 1, Neutrophil cytosol factor 1, NCF-47K, NCF47K, NCF1A, NCF1, NCF 47K, NADPH oxidase organizer 2, 47kDa neutrophil oxidase factor, 47kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, 47 kDa autosomal chronic granulomatous disease protein, ATGP0653-10 µg, ATGP0653-20 µg, ATGP0653-50 µg, ATGP0653-100 µg, ATGP0653-250 µg, ATGP0653-500 µg, ATGP0653-1 mg, ATGP0653-010, ATGP0653-020, ATGP0653-050, ATGP0653-100, ATGP0653-250, ATGP0653-500, ATGP0653-01M

Wienties FB., et al. (2001) Biochem Biophys Res Commun. 289 (2) :382-8. ; ; Doerries C., et al. (2007) Circ Res. 100:894-903.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 0.1M NaCl

Cancer

AAH02816

P14598

Human