Recombinant humaan p62/SQSTM1-eiwit

SQSTM1 is an adapter protein which binds ubiquitin and regulates signaling cascades through ubiquitination. It may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1 and play a role in titin/TTN downstream signaling in muscle cells. This protein also may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels. Mutations in the ubiquitin-associated (uBA) domain of the sequestosome 1 protein commonly cause Paget's disease since the uBA is necessary for aggregate sequestration and cell survival. Recombinant SQSTM1 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Sequestosome 1, A170, OSIL, p60, p62, p62B, PDB3, ZIP3, Sequestosome 1 EBI 3 associated protein of 60 kDa, ZIP, ZIP 3, EBI3 associated protein of 60 kDa, EBIAP, MGC127197, ORCA.M530, OSF-6, Osi, Oxidative stress induced like, Paget disease of bone 3, PDB 3, Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa, Phosphotyrosine independent ligand for the Lck SH2 domain p62, PKC-zeta-interacting protein, Protein kinase C-zeta-interacting protein, SQSTM 1, STAP, STONE14, ubiquitin binding protein p62

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

39.7 kDa (364aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

P62, SQSTM1, ATGP0532-10 µg, ATGP0532-20 µg, ATGP0532-50 µg, ATGP0532-100 µg, ATGP0532-250 µg, ATGP0532-500 µg, ATGP0532-1 mg, ATGP0532-10, ATGP0532-20, ATGP0532-50, ATGP0532-100, ATGP0532-250, ATGP0532-500, ATGP0532-1

Falchetti A., et al. (2004) J Bone Miner Res. 19 (6) :1013-7.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 1mM DTT

Signal Transduction

NP_001135771

Q13501

Human