Recombinant humaan PA28-alfa/PSME1-eiwit

PSME1 is an interferon gamma (IFNG) inducible proteasome activator required for presentation of certain major histocompatibility (MHC) class I antigens. The PSME1 complex is an alternative proteasome activator that does not employ the use of ubiquitin. The PSME1 complex is composed of two homologous subunits, alpha and beta, which have similar catalytic properties and associate to form a hexameric ring. Recombinant human PSME1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Proteasome activator subunit 1, 11S regulator complex subunit alpha, REG-alpha, Activator of multicatalytic protease subunit 1, Interferon gamma up-regulated I-5111 protein, IGUP I-5111, Proteasome activator 28 subunit alpha, PA28a, PA28alpha, IFI5111

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

30.8 kDa (269aa) confirmed by MALDI-TOF

PA28 Activator a Subunit, PSME1, Proteasome activator complex subunit 1, IFI5111, MGC8628, PA28A, PA28alpha, REGalpha, ATGP0749-10 µg, ATGP0749-20 µg, ATGP0749-50 µg, ATGP0749-100 µg, ATGP0749-250 µg, ATGP0749-500 µg, ATGP0749-1 mg, ATGP0749-10, ATGP0749-20, ATGP0749-50, ATGP0749-100, ATGP0749-250, ATGP0749-500, ATGP0749-1

Ahn K.. et al. (1996) J Biol Chem. 271 (30) :18237-42; ; Murata S., et al. (2001) EMBO J.. 20 (21) :5898-907.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Ubiquitination

NP_006254

Q06323

Human

MGSSHHHHHH SSGLVPRGSH MAMLRVQPEA QAKVDVFRED LCTKTENLLG SYFPKKISEL DAFLKEPALN EANLSNLKAP LDIPVPDPVK EKEKEERKKQ QEKEDKDEKK KGEDEDKGPP CGPVNCNEKI VVLLQRLKPE IKDVIEQLNL VTTWLQLQIP RIEDGNNFGV AVQEKVFELM TSLHTKLEGF HTQISKYFSE RGDAVTKAAK QPHVGDYRQL VHELDEAEYR DIRLMVMEIR NAYAVLYDII LKNFEKLKKP RGETKGMIY