Recombinant humaan PA28 bèta/PSME2-eiwit

PSME2, also known as proteasome (prosome, macropain) activator subunit 2, is an IFN-gamma inducible proteasome activator required for presentation of certain major histocompatibility (MHC) class I antigens. Downregulation of PSME2 results in abnormal proteasome activation and has been implicated in the development of intimal hyperplasia (IH) in animal models. Recombinant human PSME2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Proteasome activator subunit 2, 11S regulator complex subunit beta, REG-beta, Activator of multicatalytic protease subunit 2, Proteasome activator 28 subunit beta, PA28b, PA28beta

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

29.5 kDa (259aa) confirmed by MALDI-TOF

PA28 Activator b Subunit, Proteasome (prosome macropain) activator subunit 2, Proteasome (prosome, macropain) activator subunit 2, PA28B, PA28beta, REGbeta, ATGP1288-10 µg, ATGP1288-20 µg, ATGP1288-50 µg, ATGP1288-100 µg, ATGP1288-250 µg, ATGP1288-500 µg, ATGP1288-1 mg, ATGP1288-10, ATGP1288-20, ATGP1288-50, ATGP1288-100, ATGP1288-250, ATGP1288-500, ATGP1288-1

Kohda K. et al. (1998) J. Immunol. 160: 4923-4935.; ; Fabunmi R.P. et al. (2001) J. Cell. Sci. 114: 29-36

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT, 0.1mM PMSF

Ubiquitination

AAH72025.1

Q9UL46

Human

MAKPCGVRLS GEARKQVEVF RQNLFQEAEE FLYRFLPQKI IYLNQLLQED SLNVADLTSL RAPLDIPIPD PPPKDDEMET DKQEKKEVPK CGFLPGNEKV LSLLALVKPE VWTLKEKCIL VITWIQHLIP KIEDGNDFGV AIQEKVLERV NAVKTKVEAF QTTISKYFSE RGDAVAKASK ETHVMDYRAL VHERDEAAYG ELRAMVLDLR AFYAELYHII SSNLEKIVNP KGEEKPSMY