Recombinant humaan PA28 gamma/PSME3-eiwit

PSME3 (Proteasome activator complex subunit 3) belongs to the PA28 family. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. PSME3 activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. PSEM3 facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. Recombinant human PSME3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Proteasome activator subunit 3, 11S regulator complex subunit gamma, REG-gamma, Activator of multicatalytic protease subunit 3, Ki nuclear autoantigen, Proteasome activator 28 subunit gamma, PA28g, PA28gamma, Ki

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

31.7 kDa (274aa) confirmed by MALDI-TOF

PA28 Activator g Subunit, Proteasome activator complex subunit 3 isoform 1, Ki, PA28-gamma, PA28G, REG-GAMMA, ATGP1302-10 µg, ATGP1302-20 µg, ATGP1302-50 µg, ATGP1302-100 µg, ATGP1302-250 µg, ATGP1302-500 µg, ATGP1302-1 mg, ATGP1302-10, ATGP1302-20, ATGP1302-50, ATGP1302-100, ATGP1302-250, ATGP1302-500, ATGP1302-1

Wilk S., et al. (2000) Arch. Biochem. Biophys. 383:265-271; ; Zhang Z., et al. (2008) EMBO J. 27:852-864

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 40% glycerol, 200 mM NaCl

Ubiquitination

NP_005780.2

P61289

Human

MASLLKVDQE VKLKVDSFRE RITSEAEDLV ANFFPKKLLE LDSFLKEPIL NIHDLTQIHS DMNLPVPDPI LLTNSHDGLD GPTYKKRRLD ECEEAFQGTK VFVMPNGMLK SNQQLVDIIE KVKPEIRLLI EKCNTVKMWV QLLIPRIEDG NNFGVSIQEE TVAELRTVES EAASYLDQIS RYYITRAKLV SKIAKYPHVE DYRRTVTEID EKEYISLRLI ISELRNQYVT LHDMILKNIE KIKRPRSSNA ETLY