Recombinant humaan PABPN1-eiwit

PABPN1 is an abundant nuclear protein that binds with high affinity to nascent poly (A) tails. The protein is required for progressive and efficient polymerization of poly (A) tails at the 3' ends of eukaryotic transcripts and controls the size of the poly (A) tail to about 250 nt. At steady-state, this protein is localized in the nucleus whereas a different poly (A) binding protein is localized in the cytoplasm. This gene contains a GCG trinucleotide repeat at the 5' end of the coding region, and expansion of this repeat from the normal 6 copies to 8-13 copies leads to autosomal dominant oculopharyngeal muscular dystrophy (OPMD) disease. Related pseudogenes have been identified on chromosomes 19 and X. Read-through transcription also exists between this gene and the neighboring upstream BCL2-like 2 (BCL2L2) gene. Recombinant human PABPN1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Polyadenylate-binding protein 2, OPMD, PAB2, PABII, PABP-2, PABP2

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

23.8 kDa (211aa)

PABPN1, Polyadenylate-binding protein 2, OPMD, PAB2, PABII, PABP-2, PABP2, ATGP2500-10 µg, ATGP2500-20 µg, ATGP2500-50 µg, ATGP2500-100 µg, ATGP2500-250 µg, ATGP2500-500 µg, ATGP2500-1 mg, ATGP2500-10, ATGP2500-20, ATGP2500-50, ATGP2500-100, ATGP2500-250, ATGP2500-500, ATGP2500-1

Fan X., et al. (2001) Hum. Mol. Genet. 10:2341-2351.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Epigenomics (Transcription & Translation)

NP_004634

Q86U42

Human

MGSSHHHHHH SSGLVPRGSH MGSLEAIKAR VREMEEEAEK LKELQNEVEK QMNMSPPPGN AGPVIMSIEE KMEADARSIY VGNVDYGATA EELEAHFHGC GSVNRVTILC DKFSGHPKGF AYIEFSDKES VRTSLALDES LFRGRQIKVI PKRTNRPGIS TTDRGFPRAR YRARTTNYNS SRSRFYSGFN SRPRGRVYRG RARATSWYSP Y