Recombinant humaan PACT-eiwit

PRKRA is a protein kinase activated by double-stranded RNA which mediates the effects of interferon in response to viral infection. Mutations in this gene have been associated with dystonia. Alternative splicing results in multiple transcript variants. Recombinant human PRKRA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Protein kinase interferon-inducible double stranded RNA dependent activator, Protein kinase, interferon-inducible double stranded RNA dependent activator, DYT16, HSD14, PACT, RAX

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

36.8 kDa (336aa) confirmed by MALDI-TOF

PACT, Protein kinase interferon-inducible double stranded RNA dependent activator, Protein kinase, interferon-inducible double stranded RNA dependent activator, DYT16, HSD14, PACT, RAX, ATGP2775-10 µg, ATGP2775-20 µg, ATGP2775-50 µg, ATGP2775-100 µg, ATGP2775-250 µg, ATGP2775-500 µg, ATGP2775-1 mg, ATGP2775-10, ATGP2775-20, ATGP2775-50, ATGP2775-100, ATGP2775-250, ATGP2775-500, ATGP2775-1

Peters G.A., et al. (2001) Mol. Cell. Biol. 21:1908-1920; ; Peters G.A., et al. (2006) J. Biol. Chem. 281:35129-35136

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT, 1mM EDTA

Epigenomics (Transcription & Translation)

NP_003681

O75569

Human

MGSSHHHHHH SSGLVPRGSH MGSMSQSRHR AEAPPLERED SGTFSLGKMI TAKPGKTPIQ VLHEYGMKTK NIPVYECERS DVQIHVPTFT FRVTVGDITC TGEGTSKKLA KHRAAEAAIN ILKANASICF AVPDPLMPDP SKQPKNQLNP IGSLQELAIH HGWRLPEYTL SQEGGPAHKR EYTTICRLES FMETGKGASK KQAKRNAAEK FLAKFSNISP ENHISLTNVV GHSLGCTWHS LRNSPGEKIN LLKRSLLSIP NTDYIQLLSE IAKEQGFNIT YLDIDELSAN GQYQCLAELS TSPITVCHGS GISCGNAQSD AAHNALQYLK IIAERK