Recombinant humaan PAFAH1B3-eiwit

PAFAH1B3 protein belongs to the 'GDSL' lipolytic enzyme family. Acetylhydrolase is catalyzes the removal of an acetyl group from the glycerol backbone of platelet-activating factor. PAFAH1B3 is a subunit of the platelet-activating factor cetylhydrolase isoform 1B complex, which consists of the catalytic beta and gamma subunits and the regulatory alpha subunit. This complex plays an important role during the development of brain. Recombinant human PAFAH1B3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Platelet-activating factor acetylhydrolase IB subunit gamma, PAFAHG, PAF-AH1b alpha 1 subunit

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

28.2 kDa (254aa) confirmed by MALDI-TOF

PAFAH1B3, Platelet-activating factor acetylhydrolase IB subunit gamma, PAFAHG, PAF-AH1b alpha 1 subunit, ATGP1674-10 µg, ATGP1674-20 µg, ATGP1674-50 µg, ATGP1674-100 µg, ATGP1674-250 µg, ATGP1674-500 µg, ATGP1674-1 mg, ATGP1674-10, ATGP1674-20, ATGP1674-50, ATGP1674-100, ATGP1674-250, ATGP1674-500, ATGP1674-1

Moro F, et al. (1998) Genomics 51 (1) : 157-9; ; TW, Li J, et al. (2001) Protein Eng. 14 (7) : 513-9.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol

Metabolism

NP_001139411

Q15102

Human

MSGEENP ASKPTPVQDV QGDGRWMSLH HRFVADSKDK EPEVVFIGDS LVQLMHQCEI WRELFSPLHA LNFGIGGDGT QHVLWRLENG ELEHIRPKIV VVWVGTNNHG HTAEQVTGGI KAIVQLVNER QPQARVVVLG LLPRGQHPNP LREKNRQVNE LVRAALAGHP RAHFLDADPG FVHSDGTISH HDMYDYLHLS RLGYTPVCRA LHSLLLRLLA QDQGQGAPLL EPAP