Recombinant humaan PAICS-eiwit

PAICS, also known as phosphoribosylaminoimidazole carboxylase, is an enzyme involved in nucleotide biosynthesis and in particular in purine biosynthesis. It catalyzes the conversion of 5'-phosphoribosyl-5-aminoimidazole (AIR) into 5'-phosphoribosyl-4-carboxy-5-aminoimidazole (CAIR) as described in the reaction. Recombinant human PAICS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Multifunctional protein ADE2, ADE2, ADE2H1, AIRC, PAIS

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

49.5 kDa (448aa) confirmed by MALDI-TOF

PAICS, Multifunctional protein ADE2, ADE2, ADE2H1, AIRC, PAIS, ATGP2628-10 µg, ATGP2628-20 µg, ATGP2628-50 µg, ATGP2628-100 µg, ATGP2628-250 µg, ATGP2628-500 µg, ATGP2628-1 mg, ATGP2628-10, ATGP2628-20, ATGP2628-50, ATGP2628-100, ATGP2628-250, ATGP2628-500, ATGP2628-1

Ealick SE. et al. (1999) Structure. 7:1395-1406; ; Meyer E. et al. (1999) Biochemistry. 38:3012-3018.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 0.1M NaCl,1mM DTT

Epigenomics (Transcription & Translation)

NP_001072992

P22234

Human

MGSSHHHHHH SSGLVPRGSH MGSMATAEVL NIGKKLYEGK TKEVYELLDS PGKVLLQSKD QITAGNAARK NHLEGKAAIS NKITSCIFQL LQEAGIKTAF TRKCGETAFI APQCEMIPIE WVCRRIATGS FLKRNPGVKE GYKFYPPKVE LFFKDDANND PQWSEEQLIA AKFCFAGLLI GQTEVDIMSH ATQAIFEILE KSWLPQNCTL VDMKIEFGVD VTTKEIVLAD VIDNDSWRLW PSGDRSQQKD KQSYRDLKEV TPEGLQMVKK NFEWVAERVE LLLKSESQCR VVVLMGSTSD LGHCEKIKKA CGNFGIPCEL RVTSAHKGPD ETLRIKAEYE GDGIPTVFVA VAGRSNGLGP VMSGNTAYPV ISCPPLTPDW GVQDVWSSLR LPSGLGCSTV LSPEGSAQFA AQIFGLSNHL VWSKLRASIL NTWISLKQAD KKIRECNL