Recombinant humaan PAIP2-eiwit

PAIP2, also known as Poly (A) -binding protein-interacting protein 2, acts as a repressor in the regulation of translation initiation of poly (A) -containing mRNAs. Its inhibitory activity on translation is mediated via its action on PABPC1. It displaces the interaction of PABPC1 with poly (A) RNA and competes with PAIP1 for binding to PABPC1. Its association with PABPC1 results in disruption of the cytoplasmic poly (A) RNP structure organization. Recombinant PAIP2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Poly (A) -binding protein-interacting protein 2, PAIP2A, PABP- interacting protein 2, HSPC218, Poly (A) -binding protein-interacting protein 2 poly (A) binding protein interacting protein 2.

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

17.1 kDa (147aa) confirmed by MALDI-TOF

PAIP2, Poly (A) -binding protein-interacting protein 2, PAIP2A, PABP- interacting protein 2, HSPC218, Poly (A) -binding protein-interacting protein 2 poly (A) binding protein interacting protein 2., ATGP0416-10 µg, ATGP0416-20 µg, ATGP0416-50 µg, ATGP0416-100 µg, ATGP0416-250 µg, ATGP0416-500 µg, ATGP0416-1 mg, ATGP0416-10, ATGP0416-20, ATGP0416-50, ATGP0416-100, ATGP0416-250, ATGP0416-500, ATGP0416-1

Siddiqui N., et al. (2007) J Biol Chem. 282 (34) :25067-75. ; ; Yoshida M., et al. (2006) EMBO J. 25 (9) :1934-44.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Metabolism

NP_057564

Q9BPZ3

Human

MKDPSRSSTS PSIINEDVII NGHSHEDDNP FAEYMWMENE EEFNRQIEEE LWEEEFIERC FQEMLEEEEE HEWFIPARDL PQTMDQIQDQ FNDLVISDGS SLEDLVVKSN LNPNAKEFVP GVKYGNI