Recombinant humaan PALM-eiwit

PALM is a member of the paralemmin protein family. The product of this gene is a prenylated and palmitoylated phosphoprotein that associates with the cytoplasmic face of plasma membranes and is implicated in plasma membrane dynamics in neurons and other cell types. Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined. Recombinant human PALM protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Paralemmin-1 isoform 1, Paralemmin

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

44.5 kDa (410aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

PALM, Paralemmin-1 isoform 1, Paralemmin, ATGP2770-10 µg, ATGP2770-20 µg, ATGP2770-50 µg, ATGP2770-100 µg, ATGP2770-250 µg, ATGP2770-500 µg, ATGP2770-1 mg, ATGP2770-10, ATGP2770-20, ATGP2770-50, ATGP2770-100, ATGP2770-250, ATGP2770-500, ATGP2770-1

Gauthier-Campbell C., Bredt D.S., et al. (2004) Mol. Biol. Cell 15:2205-2217

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Neuroscience

NP_002570

O75781

Human

MGSSHHHHHH SSGLVPRGSH MGSMEVLAAE TTSQQERLQA IAEKRKRQAE IENKRRQLED ERRQLQHLKS KALRERWLLE GTPSSASEGD EDLRRQMQDD EQKTRLLEDS VSRLEKEIEV LERGDSAPAT AKENAAAPSP VRAPAPSPAK EERKTEVVMN SQQTPVGTPK DKRVSNTPLR TVDGSPMMKA AMYSVEITVE KDKVTGETRV LSSTTLLPRQ PLPLGIKVYE DETKVVHAVD GTAENGIHPL SSSEVDELIH KADEVTLSEA GSTAGAAETR GAVEGAARTT PSRREITGVQ AQPGEATSGP PGIQPGQEPP VTMIFMGYQN VEDEAETKKV LGLQDTITAE LVVIEDAAEP KEPAPPNGSA AEPPTEAASR EENQAGPEAT TSDPQDLDMK KHRCKCCSIM