Recombinant humaan PAPSS1-eiwit

PAPSS1, also known as bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1, is a bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway, yielding 3'-phosphoadenylylsulfate (PAPS) . PAPSS1 is also involved in the biosynthesis of sulfated L-selectin ligands in endothelial cells. Recombinant human PAPSS1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1, ATPSK1, PAPSS, SK1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

70.9 kDa (626aa)

PAPSS1, Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1, ATPSK1, PAPSS, SK1, ATGP1390-10 µg, ATGP1390-20 µg, ATGP1390-50 µg, ATGP1390-100 µg, ATGP1390-250 µg, ATGP1390-500 µg, ATGP1390-1 mg, ATGP1390-10, ATGP1390-20, ATGP1390-50, ATGP1390-100, ATGP1390-250, ATGP1390-500, ATGP1390-1

Girard J.-P., et al. (1998) FASEB J. 12:603-612; ; Venkatachalam K.V., et al. (1999) J. Biol. Chem. 274:2601-2604

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl

Enzymes & Proteases

NP_005434

O43252

Human

RATNV TYQAHHVSRN KRGQVVGTRG GFRGCTVWLT GLSGAGKTTV SMALEEYLVC HGIPCYTLDG DNIRQGLNKN LGFSPEDREE NVRRIAEVAK LFADAGLVCI TSFISPYTQD RNNARQIHEG ASLPFFEVFV DAPLHVCEQR DVKGLYKKAR AGEIKGFTGI DSEYEKPEAP ELVLKTDSCD VNDCVQQVVE LLQERDIVPV DASYEVKELY VPENKLHLAK TDAETLPALK INKVDMQWVQ VLAEGWATPL NGFMREREYL QCLHFDCLLD GGVINLSVPI VLTATHEDKE RLDGCTAFAL MYEGRRVAIL RNPEFFEHRK EERCARQWGT TCKNHPYIKM VMEQGDWLIG GDLQVLDRVY WNDGLDQYRL TPTELKQKFK DMNADAVFAF QLRNPVHNGH ALLMQDTHKQ LLERGYRRPV LLLHPLGGWT KDDDVPLMWR MKQHAAVLEE GVLNPETTVV AIFPSPMMYA GPTEVQWHCR ARMVAGANFY IVGRDPAGMP HPETGKDLYE PSHGAKVLTM APGLITLEIV PFRVAAYNKK KKRMDYYDSE HHEDFEFISG TRMRKLAREG QKPPEGFMAP KAWTVLTEYY KSLEKA