Recombinant humaan PARD6B-eiwit

Partitioning defective 6 homolog beta, also known as PARD6B, is a member of the PAR6 family and encodes a protein with a PSD95/Discs-large/ZO1 (PDZ) domain, an OPR domain and a semi-Cdc42/Rac interactive binding (CRIB) domain. Cellular asymmetry is critical for the development of multicellular organisms. PARD (partitioning-defective) proteins play important roles in asymmetric cell division and polarized growth, whereas Cdc42 and Rac mediate establishment of cell growth and polarity and contribute to oncogenic transformation by Ras. It is expressed in pancreas and in both adult and fetal kidney, and is weakly expressed in placenta and lung. Recombinant human PARD6B protein, fused to His-tag at N-terminus, was expressed in E. coli.

Partitioning defective 6 homolog beta, PAR6B

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

43.6 kDa (395aa)

PARD6B, Partitioning defective 6 homolog beta, PAR6B, ATGP1840-10 µg, ATGP1840-20 µg, ATGP1840-50 µg, ATGP1840-100 µg, ATGP1840-250 µg, ATGP1840-500 µg, ATGP1840-1 mg, ATGP1840-10, ATGP1840-20, ATGP1840-50, ATGP1840-100, ATGP1840-250, ATGP1840-500, ATGP1840-1

Joberty G., et al. (2000) Nat Cell Biol. 2:531-539.; ; Qiu R G., et al. (2000) Curr Biol. 10:697-707.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2M urea, 20% glycerol, 0.2M NaCl

Cell Cycle

NP_115910

Q9BYG5

Human

MGSSHHHHHH SSGLVPRGSH MGSMNRSHRH GAGSGCLGTM EVKSKFGAEF RRFSLERSKP GKFEEFYGLL QHVHKIPNVD VLVGYADIHG DLLPINNDDN YHKAVSTANP LLRIFIQKKE EADYSAFGTD TLIKKKNVLT NVLRPDNHRK KPHIVISMPQ DFRPVSSIID VDILPETHRR VRLYKYGTEK PLGFYIRDGS SVRVTPHGLE KVPGIFISRL VPGGLAQSTG LLAVNDEVLE VNGIEVSGKS LDQVTDMMIA NSRNLIITVR PANQRNNVVR NSRTSGSSGQ STDNSLLGYP QQIEPSFEPE DEDSEEDDII IEDNGVPQQI PKAVPNTESL ESLTQIELSF ESGQNGFIPS NEVSLAAIAS SSNTEFETHA PDQKLLEEDG TIITL