Recombinant humaan Parkin-eiwit

The precise function of this gene is unknown; however, the encoded protein is a component of a multiprotein E3 ubiquitin ligase complex that mediates the targeting of substrate proteins for proteasomal degradation. Mutations in this gene are known to cause Parkinson disease and autosomal recessive juvenile Parkinson disease. Alternative splicing of this gene produces multiple transcript variants encoding distinct isoforms. Additional splice variants of this gene have been described but currently lack transcript support. Recombinant human PARK2 protein, fused to His-tag at N-terminus, was expressed in E. coli.

E3 ubiquitin-protein ligase parkin, AR-JP, LPRS2, PDJ, PRKN

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

53.8 kDa (485aa)

Parkin, E3 ubiquitin-protein ligase parkin, AR-JP, LPRS2, PDJ, PRKN, ATGP2517-10 µg, ATGP2517-20 µg, ATGP2517-50 µg, ATGP2517-100 µg, ATGP2517-250 µg, ATGP2517-500 µg, ATGP2517-1 mg, ATGP2517-10, ATGP2517-20, ATGP2517-50, ATGP2517-100, ATGP2517-250, ATGP2517-500, ATGP2517-1

Imai Y., et al. (2000) J. Biol. Chem. 275:35661-35664; ; Shimura H., et al. (2000) Nat. Genet. 25:302-305;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Metabolism

NP_004553.2

O60260

Human

MGSSHHHHHH SSGLVPRGSH MIVFVRFNSS HGFPVEVDSD TSIFQLKEVV AKRQGVPADQ LRVIFAGKEL RNDWTVQNCD LDQQSIVHIV QRPWRKGQEM NATGGDDPRN AAGGCEREPQ SLTRVDLSSS VLPGDSVGLA VILHTDSRKD SPPAGSPAGR SIYNSFYVYC KGPCQRVQPG KLRVQCSTCR QATLTLTQGP SCWDDVLIPN RMSGECQSPH CPGTSAEFFF KCGAHPTSDK ETSVALHLIA TNSRNITCIT CTDVRSPVLV FQCNSRHVIC LDCFHLYCVT RLNDRQFVHD PQLGYSLPCV AGCPNSLIKE LHHFRILGEE QYNRYQQYGA EECVLQMGGV LCPRPGCGAG LLPEPDQRKV TCEGGNGLGC GFAFCRECKE AYHEGECSAV FEASGTTTQA YRVDERAAEQ ARWEAASKET IKKTTKPCPR CHVPVEKNGG CMHMKCPQPQ CRLEWCWNCG CEWNRVCMGD HWFDV