Recombinant humaan PBK-eiwit

Lymphokine-activated killer T-cell-originated protein kinase, also known as PBK, is a serine/threonine kinase related to the dual specific mitogen-activated protein kinase (MAPKK) family. PBK is abundant in placenta and absent from adult brain tissue. A PDZ domain in the tumor suppressor protein Dlg can coordinate with the T/SXV motif of PBK. This protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Recombinant human PBK protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Lymphokine-activated killer T-cell-originated protein kinase, CT84, Nori-3, SPK, TOPK

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

38.6 kDa (346aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

PBK, Lymphokine-activated killer T-cell-originated protein kinase, CT84, Nori-3, SPK, TOPK, ATGP1437-10 µg, ATGP1437-20 µg, ATGP1437-50 µg, ATGP1437-100 µg, ATGP1437-250 µg, ATGP1437-500 µg, ATGP1437-1 mg, ATGP1437-10, ATGP1437-20, ATGP1437-50, ATGP1437-100, ATGP1437-250, ATGP1437-500, ATGP1437-1

Abe Y., et al. (2000) J Biol Chem. 275:21525-21531.; ; Dougherty J D., et al. (2005) J Neurosci. 25:10773-10785.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Cell Cycle

NP_060962

Q96KB5

Human

MGSSHHHHHH SSGLVPRGSH MGSHMEGISN FKTPSKLSEK KKSVLCSTPT INIPASPFMQ KLGFGTGVNV YLMKRSPRGL SHSPWAVKKI NPICNDHYRS VYQKRLMDEA KILKSLHHPN IVGYRAFTEA NDGSLCLAME YGGEKSLNDL IEERYKASQD PFPAAIILKV ALNMARGLKY LHQEKKLLHG DIKSSNVVIK GDFETIKICD VGVSLPLDEN MTVTDPEACY IGTEPWKPKE AVEENGVITD KADIFAFGLT LWEMMTLSIP HINLSNDDDD EDKTFDESDF DDEAYYAALG TRPPINMEEL DESYQKVIEL FSVCTNEDPK DRPSAAHIVE ALETDV