Recombinant humaan PBLD-eiwit

PBLD, also known as MAWBP, is a phenazine biosynthesis-like protein (PhzF) family. Expressed in most tissues, PBLD is the only representative of the PhzF family in the human genome. It is participate in the MAPK signaling pathway. Involved in multiple basic cellular functions, expression of PBLD is elevated in several disease processes, including Insulin resistance, folate deficiency and hypotension. Recombinant human PBLD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Phenazine biosynthesis-like domain-containing protein, FLJ14767, MAWBP, MAWDBP.

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

33.9 kDa (308aa) confirmed by MALDI-TOF

PBLD, Phenazine biosynthesis-like domain-containing protein, FLJ14767, MAWBP, MAWDBP., ATGP0790-10 µg, ATGP0790-20 µg, ATGP0790-50 µg, ATGP0790-100 µg, ATGP0790-250 µg, ATGP0790-500 µg, ATGP0790-1 mg, ATGP0790-10, ATGP0790-20, ATGP0790-50, ATGP0790-100, ATGP0790-250, ATGP0790-500, ATGP0790-1

Mavrodi DV., et al. (2004) Acta Crystallogr D Biol Crystallogr. 60 (1) :184-6. ; ; Parsons JF., et al. (2004) Biochemistry. 43 (39) :12427-35.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.

Enzymes & Proteases

NP_071412

P30039

Human

MGSSHHHHHH SSGLVPRGSH MKLPIFIADA FTARAFRGNP AAVCLLENEL DEDMHQKIAR EMNLSETAFI RKLHPTDNFA QSSCFGLRWF TPASEVPLCG HATLASAAVL FHKIKNMNST LTFVTLSGEL RARRAEDGIV LDLPLYPAHP QDFHEVEDLI KTAIGNTLVQ DICYSPDTQK LLVRLSDVYN RSFLENLKVN TENLLQVENT GKVKGLILTL KGEPGGQTQA FDFYSRYFAP WVGVAEDPVT GSAHAVLSSY WSQHLGKKEM HAFQCSHRGG ELGISLRPDG RVDIRGGAAV VLEGTLTA