Recombinant humaan PCBD1-eiwit

Pterin-4-alpha-carbinolamine dehydratase precursor, also known as PCBD1, is a component of the phenylalanine hydroxylase (PAH) system and participates in tetrahydrobiopterin biosynthesis. A deficiency of this enzyme leads to hyperphenylalaninemia. The enzyme regulates the homodimerization of the transcription factor hepatocyte nuclear factor 1 (HNF1) . Recombinant human PCBD1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Pterin-4-alpha-carbinolamine dehydratase, DCOH, PCBD, PCD, PHS

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

14.1 kDa (124aa) confirmed by MALDI-TOF

PCBD1, Pterin-4-alpha-carbinolamine dehydratase, DCOH, PCBD, PCD, PHS, ATGP0699-10 µg, ATGP0699-20 µg, ATGP0699-50 µg, ATGP0699-100 µg, ATGP0699-250 µg, ATGP0699-500 µg, ATGP0699-1 mg, ATGP0699-10, ATGP0699-20, ATGP0699-50, ATGP0699-100, ATGP0699-250, ATGP0699-500, ATGP0699-1

Schallreuter K u., et al. (2004) Biochem Biophys Res Commun. 315:502-508.; ; Hasse S., et al. (2005) Exp Dermatol. 14:182-187.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Metabolism

NP_000272

P61457

Human

MAGKAHRLSA EERDQLLPNL RAVGWNELEG RDAIFKQFHF KDFNRAFGFM TRVALQAEKL DHHPEWFNVY NKVHITLSTH ECAGLSERDI NLASFIEQVA VSMT