Recombinant humaan PCK1-eiwit

Phosphoenolpyruvate carboxykinase 1, also known as PCK1, is a main control point for the regulation of gluconeogenesis. PCK1 plays an important role in this process by stimulating hepatic glucose production. The expression of PCK1 can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Modulation of the signals governing PCK1 levels presents a potential therapeutic approach to the treatment of Insulin resistance and consequently obesity. Recombinant human PCK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Phosphoenolpyruvate carboxykinase 1, Phosphoenolpyruvate carboxykinase 1, PEPCK-C, PEPCK1, PEPCKC

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

71.7 kDa (646aa)

PCK1, Phosphoenolpyruvate carboxykinase 1, Phosphoenolpyruvate carboxykinase 1, PEPCK-C, PEPCK1, PEPCKC, ATGP1372-10 µg, ATGP1372-20 µg, ATGP1372-50 µg, ATGP1372-100 µg, ATGP1372-250 µg, ATGP1372-500 µg, ATGP1372-1 mg, ATGP1372-10, ATGP1372-20, ATGP1372-50, ATGP1372-100, ATGP1372-250, ATGP1372-500, ATGP1372-1

Barthel A., et al. (2003) Am J Physiol Endocrinol Metab. 285:685-692.; ; Wang Y., et al. (1991) J Cell Physiol. 147:374-382.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Metabolism

AAH23978.1

P35558

Human

MPPQLQ NGLNLSAKVV QGSLDSLPQA VREFLENNAE LCQPDHIHIC DGSEEENGRL LGQMEEEGIL RRLKKYDNCW LALTDPRDVA RIESKTVIVT QEQRDTVPIP KTGLSQLGRW MSEEDFEKAF NARFPGCMKG RTMYVIPFSM GPLGSPLSKI GIELTDSPYV VASMRIMTRM GTPVLEALGD GEFVKCLHSV GCPLPLQKPL VNNWPCNPEL TLIAHLPDRR EIISFGSGYG GNSLLGKKCF ALRMASRLAK EEGWLAEHML VLGITNPEGE KKYLAAAFPS ACGKTNLAMM NPSLPGWKVE CVGDDIAWMK FDAQGHLRAI NPENGFFGVA PGTSVKTNPN AIKTIQKNTI FTNVAETSDG GVYWEGIDEP LASGVTITSW KNKEWSSEDG EPCAHPNSRF CTPASQCPII DAAWESPEGV PIEGIIFGGR RPAGVPLVYE ALSWQHGVFV GAAMRSEATA AAEHKGKIIM HDPFAMRPFF GYNFGKYLAH WLSMAQHPAA KLPKIFHVNW FRKDKEGKFL WPGFGENSRV LEWMFNRIDG KASTKLTPIG YIPKEDALNL KGLGHINMME LFSISKEFWE KEVEDIEKYL EDQVNADLPC EIEREILALK QRISQM