Recombinant humaan PCMT1-eiwit

PCMT1, also known as L-isoaspartyl protein carboxyl methyltransferase, is an enzyme that catalyses the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. This protein plays a role in the repair and/or degradation of damaged proteins. Recombinant human PCMT1 protien, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Protein-L-isoaspartate D-aspartate O-methyltransferase, PIMT, L-isoaspartyl protein carboxyl methyltransferase, Protein L-isoaspartyl/D-aspartyl methyltransferase, Protein-beta-aspartate methyltransferase

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

28.7 kDa (263aa) confirmed by MALDI-TOF

PCMT, Protein-L-isoaspartate O-methyltransferase, Protein-L-isoaspartate O-methyltransferase L isoaspartyl protein carboxyl methyltransferase, L isoaspartyl/D aspartyl methyltransferase, PCMT 1, PIMT, Protein beta aspartate methyltransferase, Protein L isoaspartate (D aspartate) O methyltransferase, Protein L isoaspartyl/D aspartyl methyltransferase, ATGP0578-10 µg, ATGP0578-20 µg, ATGP0578-50 µg, ATGP0578-100 µg, ATGP0578-250 µg, ATGP0578-500 µg, ATGP0578-1 mg, ATGP0578-10, ATGP0578-20, ATGP0578-50, ATGP0578-100, ATGP0578-250, ATGP0578-500, ATGP0578-1

Kagan RM., et al. (1997) Comp Biochem Physiol B Biochem Mol Biol. 117 (3) :379-85.

Maeda H, et al. Rapid survey of four Asp isomers in disease-related proteins by LC-MS combined with commercial enzymes. (Anal Chem. 2015) {25479244}; ; Lyon YA, et al. Differences in α-Crystallin isomerization reveal the activity of protein isoaspartyl methyltransferase (PIMT) in the nucleus and cortex of human lenses. (Exp Eye Res. 2018) {https://pubmed.ncbi.nlm.nih.gov/29571628/}; ; Takata T, et al. Site-specific rapid deamidation and isomerization in human lens αA-crystallin in vitro. (Protein Sci. 2020) {https://pubmed.ncbi.nlm.nih.gov/31930615/}; ; Magami K, et al. Isomerization of Asp is essential for assembly of amyloid-like fibrils of αA-crystallin-derived peptide. (PLoS One. 2021) {https://pubmed.ncbi.nlm.nih.gov/33857260/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl

Enzymes & Proteases

AAH08748.1

P22061

Human

MAWK SGGASHSELI HNLRKNGIIK TDKVFEVMLA TDRSHYAKCN PYMDSPQSIG FQATISAPHM HAYALELLFD QLHEGAKALD VGSGSGILTA CFARMVGCTG KVIGIDHIKE LVDDSINNVR KDDPTLLSSG RVQLVVGDGR MGYAEEAPYD AIHVGAAAPV VPQALIDQLK PGGRLILPVG PAGGNQMLEQ YDKLQDGSIK MKPLMGVIYV PLTDKEKQWS RWK