Recombinant humaan PCNP-eiwit

PCNP (PEST proteolytic signal containing nuclear protein) is a nuclear protein implied to play a role in cell cycle regulation and tumorigenesis. This protein is ubiquitinated post-translationally by NIRF, an ubiquitin ligase. PCNP exsists as three isoforms produced by alternative splicing events and may be involved in cell cycle regulation. Recombinant human PCNP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

PEST proteolytic signal containing nuclear protein., DKFZp781I24156

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by BCA assay)

> 85% by SDS-PAGE

21.5 kDa (202aa) confirmed by MALDI-TOF

PCNP, PEST proteolytic signal containing nuclear protein., DKFZp781I24156, ATGP1171-10 µg, ATGP1171-20 µg, ATGP1171-50 µg, ATGP1171-100 µg, ATGP1171-250 µg, ATGP1171-500 µg, ATGP1171-1 mg, ATGP1171-10, ATGP1171-20, ATGP1171-50, ATGP1171-100, ATGP1171-250, ATGP1171-500, ATGP1171-1

Mori T., et al. (2004) FEBS Lett. 557 (1-3) :209-14.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 10% glycerol

Cell Cycle

NP_065090.1

Q8WW12

Human

MADGKA GDEKPEKSQR AGAAGGPEEE AEKPVKTKTV SSSNGGESSS RSAEKRSAEE EAADLPTKPT KISKFGFAIG SQTTKKASAI SIKLGSSKPK ETVPTLAPKT LSVAAAFNED EDSEPEEMPP EAKMRMKNIG RDTPTSAGPN SFNKGKHGFS DNQKLWERNI KSHLGNVHDQ DN