Recombinant humaan PCPBP1-eiwit

PCBP1 appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly (rC) -binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promotes poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. PCBP1 is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. Recombinant human PCBP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Poly (rC) binding protein 1, Poly (rC) binding protein 1, hnRNP-E1, hnRNP-X, HNRPE1, HNRPX

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

19.6 kDa (186aa) confirmed by MALDI-TOF

PCBP1, Poly (rC) binding protein 1, Poly (rC) binding protein 1, hnRNP-E1, hnRNP-X, HNRPE1, HNRPX, ATGP2314-10 µg, ATGP2314-20 µg, ATGP2314-50 µg, ATGP2314-100 µg, ATGP2314-250 µg, ATGP2314-500 µg, ATGP2314-1 mg, ATGP2314-10, ATGP2314-20, ATGP2314-50, ATGP2314-100, ATGP2314-250, ATGP2314-500, ATGP2314-1

Beausoleil S.A., et al. (2006) Nat. Biotechnol. 24:1285-1292; ; Yu L.-R., et al. (2007) J. Proteome Res. 6:4150-4162;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_006187.2

Q15365

Human

MDAGVTE SGLNVTLTIR LLMHGKEVGS IIGKKGESVK RIREESGARI NISEGNCPER IITLTGPTNA IFKAFAMIID KLEEDINSSM TNSTAASRPP VTLRLVVPAT QCGSLIGKGG CKIKEIREST GAQVQVAGDM LPNSTERAIT IAGVPQSVTE CVKQIC