Recombinant humaan PCYT2-eiwit

PCYT2 is an enzyme that catalyzes the formation of CDP-ethanolamine from CTP and phosphoethanolamine in the Kennedy pathway of phospholipid synthesis. Alternative splicing results in multiple transcript variants. Recombinant human PCYT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Ethanolamine-phosphate cytidylyltransferase isoform 2, CTP:phosphoethanolamine cytidylyltransferase, ET, Ethanolamine-phosphate cytidylyltransferase, Phosphorylethanolamine transferase

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

45.9 kDa (409aa) confirmed by MALDI-TOF

PCYT2, Ethanolamine-phosphate cytidylyltransferase isoform 2, CTP:phosphoethanolamine cytidylyltransferase, ET, Ethanolamine-phosphate cytidylyltransferase, Phosphorylethanolamine transferase, ATGP1348-10 µg, ATGP1348-20 µg, ATGP1348-50 µg, ATGP1348-100 µg, ATGP1348-250 µg, ATGP1348-500 µg, ATGP1348-1 mg, ATGP1348-10, ATGP1348-20, ATGP1348-50, ATGP1348-100, ATGP1348-250, ATGP1348-500, ATGP1348-1

Nakashima A. et al. (1997) J. Biol. Chem. 272: 9567-9572.; ; Bakovic M. et al. (2007) Biochem. Cell Biol. 85:283-300.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT, 0.1mM PMSF

Neuroscience

NP_002852

Q99447

Human

MGSSHHHHHH SSGLVPRGSH MIRNGRGAAG GAEQPGPGGR RAVRVWCDGC YDMVHYGHSN QLRQARAMGD YLIVGVHTDE EIAKHKGPPV FTQEERYKMV QAIKWVDEVV PAAPYVTTLE TLDKYNCDFC VHGNDITLTV DGRDTYEEVK QAGRYRECKR TQGVSTTDLV GRMLLVTKAH HSSQEMSSEY REYADSFGKC PGGRNPWTGV SQFLQTSQKI IQFASGKEPQ PGETVIYVAG AFDLFHIGHV DFLEKVHRLA ERPYIIAGLH FDQEVNHYKG KNYPIMNLHE RTLSVLACRY VSEVVIGAPY AVTAELLSHF KVDLVCHGKT EIIPDRDGSD PYQEPKRRGI FRQIDSGSNL TTDLIVQRII TNRLEYEARN QKKEAKELAF LEAARQQAAQ PLGERDGDF