Recombinant humaan PD-L1/B7-H1-eiwit

CD274, also known as programmed cell death 1 ligand 1 isoform, is a member of the growing B7 family of immune molecules. This protein is involved in the regulation of cellular and humoral immune responses. It suppresses T cell activation and proliferation and induces the apoptosis of activated T cells. Also, it plays a role in the development of immune tolerance by promoting T cell anergy and enhancing regulatory T cell development. Recombinant human CD274, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Human PD-1 (CAT# ATGP4027) is coated at 5 µg/mL (100 ul/well) can bind Human PD-L1. The ED50 range ≤ 0.5 µg/mL.

Programmed cell death 1 ligand 1 isoform, CD274, B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1

Baculovirus

Human

HIgG-His-Tag

SDS-PAGE, Bioactivity

1 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Measured by its binding ability in a functional ELISA with Human PD-1 (CAT# ATGP4027) . The ED50 range ≤ 0.5 µg/mL.

52.5 kDa (462aa)

PD-L1, B7-H1, Programmed cell death 1 ligand 1 isoform, CD274, B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, ATGP3631-10 µg, ATGP3631-20 µg, ATGP3631-50 µg, ATGP3631-100 µg, ATGP3631-250 µg, ATGP3631-500 µg, ATGP3631-1 mg, ATGP3631-10, ATGP3631-20, ATGP3631-50, ATGP3631-100, ATGP3631-250, ATGP3631-500, ATGP3631-1

Dong H., et al, (1999) Nat. Med. 5:1365-1369.; ; Trabattoni D., et al, (2003) Blood 101:2514-2520.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_054862.1

Q9NZQ7

Human

Ala

FTVTVPK DLYVVEYGSN MTIECKFPVE KQLDLAALIV YWEMEDKNII QFVHGEEDLK VQHSSYRQRA RLLKDQLSLG NAALQITDVK LQDAGVYRCM ISYGGADYKR ITVKVNAPYN KINQRILVVD PVTSEHELTC QAEGYPKAEV IWTSSDHQVL SGKTTTTNSK REEKLFNVTS TLRINTTTNE IFYCTFRRLD PEENHTAELV IPELPLAHPP NER