Recombinant humaan PD-L1/B7-H1-eiwit (CD274)

CD274, also known as programmed cell death 1 ligand 1 (PDCD1L1), is involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. This protein interaction with PDCD1 inhibits T-cell proliferation and cytokine production. Recombinant human CD274 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Programmed cell death 1 ligand, B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

27.9 kDa (245aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

PD-L1, B7-H1, Programmed cell death 1 ligand, B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, ATGP1543-10 µg, ATGP1543-20 µg, ATGP1543-50 µg, ATGP1543-100 µg, ATGP1543-250 µg, ATGP1543-500 µg, ATGP1543-1 mg, ATGP1543-10, ATGP1543-20, ATGP1543-50, ATGP1543-100, ATGP1543-250, ATGP1543-500, ATGP1543-1

Dong H., et al. (1999) Nat. Med. 5:1365-1369; ; Freeman G.J., et al. (2000) J. Exp. Med. 192:1027-1034

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Immunology

NP_054862

Q9NZQ7

Human

FTVTV PKDLYVVEYG SNMTIECKFP VEKQLDLAAL IVYWEMEDKN IIQFVHGEED LKVQHSSYRQ RARLLKDQLS LGNAALQITD VKLQDAGVYR CMISYGGADY KRITVKVNAP YNKINQRILV VDPVTSEHEL TCQAEGYPKA EVIWTSSDHQ VLSGKTTTTN SKREEKLFNV TSTLRINTTT NEIFYCTFRR LDPEENHTAE LVIPELPLAH PPNER