Recombinant humaan PDCD6IP-eiwit

Alix, also known as PDCD6IP, is a cytoplasmic protein that interacts with apoptosis-associated proteins (ALG-2 and PDCD6) and with the endocytosis-regulator CIN85. It's involved in concentration and sorting of cargo proteins of the multivesicular body for incorporation into intralumenal vesicles that are generated by invagination and scission from the limiting membrane of the endosome. Overexpression of this protein and endophilins results in cytoplasmic vacuolization which may be partly responsible for the protection against cell death. Recombinant PDCD6IP protein was expressed in E. coli and purified by using conventional chromatography techniques.

Programmed cell death 6 interacting protein, PDCD6-interacting protein, ALG-2 interacting protein X, ALG-2-interacting protein 1, Alix, AIP1, Hp95

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

45.8 kDa (412aa) confirmed by MALDI-TOF

Alix, ATGP0482-10 µg, ATGP0482-20 µg, ATGP0482-50 µg, ATGP0482-100 µg, ATGP0482-250 µg, ATGP0482-500 µg, ATGP0482-1 mg, ATGP0482-10, ATGP0482-20, ATGP0482-50, ATGP0482-100, ATGP0482-250, ATGP0482-500, ATGP0482-1

Morita E., et al. (2007) EMBO J. 26 (19) :4215-27. ; ; Katoh K., et al. (2004) Arch Biochem Biophys. 421 (1) :159-65.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 1mM DTT

Apoptosis

NP_037506.2

Q8WUM4

Human

MATFISVQLK KTSEVDLAKP LVKFIQQTYP SGGEEQAQYC RAAEELSKLR RAAVGRPLDK HEGALETLLR YYDQICSIEP KFPFSENQIC LTFTWKDAFD KGSLFGGSVK LALASLGYEK SCVLFNCAAL ASQIAAEQNL DNDEGLKIAA KHYQFASGAF LHIKETVLSA LSREPTVDIS PDTVGTLSLI MLAQAQEVFF LKATRDKMKD AIIAKLANQA ADYFGDAFKQ CQYKDTLPKE VFPVLAAKHC IMQANAEYHQ SILAKQQKKF GEEIARLQHA AELIKTVASR YDEYVNVKDF SDKINRALAA AKKDNDFIYH DRVPDLKDLD PIGKATLVKS TPVNVPISQK FTDLFEKMVP VSVQQSLAAY NQRKADLVNR SIAQMREATT LA