Recombinant humaan PDCL-eiwit

PDCL, also known as phosducin-like protein, belongs to the phosducin family. Phosducin-like protein is a putative modulator of heterotrimeric G proteins. The protein shares extensive amino acid sequence homology with phosducin, a phosphoprotein expressed in retina and pineal gland. Both phosducin-like protein and phosphoducin have been shown to regulate G-protein signaling by binding to the beta-gamma subunits of G proteins. Recombinant human PDCL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Phosducin-like protein, PhLP

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

36.8 kDa (325aa) confirmed by MALDI-TOF

PDCL, Phosducin-like protein, PhLP, ATGP1602-10 µg, ATGP1602-20 µg, ATGP1602-50 µg, ATGP1602-100 µg, ATGP1602-250 µg, ATGP1602-500 µg, ATGP1602-1 mg, ATGP1602-10, ATGP1602-20, ATGP1602-50, ATGP1602-100, ATGP1602-250, ATGP1602-500, ATGP1602-1

Thibault C., et al. (1999) Biochim. Biophys. Acta. 1444:346-354; ; Olsen J.V., et al. (2006) Cell. 127:635-648

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Signal Transduction

NP_005379

Q13371

Human

MGSSHHHHHH SSGLVPRGSH MGSHMTTLDD KLLGEKLQYY YSSSEDEDSD HEDKDRGRCA PASSSVPAEA ELAGEGISVN TGPKGVINDW RRFKQLETEQ REEQCREMER LIKKLSMTCR SHLDEEEEQQ KQKDLQEKIS GKMTLKEFAI MNEDQDDEEF LQQYRKQRME EMRQQLHKGP QFKQVFEISS GEGFLDMIDK EQKSIVIMVH IYEDGIPGTE AMNGCMICLA AEYPAVKFCK VKSSVIGASS QFTRNALPAL LIYKGGELIG NFVRVTDQLG DDFFAVDLEA FLQEFGLLPE KEVLVLTSVR NSATCHSEDS DLEID