Recombinant humaan PDE6D-eiwit

Human PDE6D was originally identified as a fourth subunit of rod-specific cGMP phosphodiesterase, PDE6. Catalytically active PDE6 is a heterodimer (alphabeta) that is regulated by two inhibitory gamma subunits. As PDE6D does not modify the catalytic properties of PDEalphabeta, its function is still unresolved. Recombinant human PDE6D, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Retinal rod rhodopsin-sensitive cGMP 3'5'-cyclic phosphodiesterase subunit delta, PDED, Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta, phosphodiesterase 6D, cGMP-specific, rod, delta

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

18.4 kDa (158aa) confirmed by MALDI-TOF

PDE6D, Retinal rod rhodopsin-sensitive cGMP 3'5'-cyclic phosphodiesterase subunit delta, PDED, Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta, phosphodiesterase 6D, cGMP-specific, rod, delta, ATGP0553-10 µg, ATGP0553-20 µg, ATGP0553-50 µg, ATGP0553-100 µg, ATGP0553-250 µg, ATGP0553-500 µg, ATGP0553-1 mg, ATGP0553-10, ATGP0553-20, ATGP0553-50, ATGP0553-100, ATGP0553-250, ATGP0553-500, ATGP0553-1

Zhang H., et al, (2007) Proc Natl Acad Sci u S A. 104 (21) :8857-62.; ; Dekomien., G. et al. (2003) Genet Sel Evol. 35 (4) :445-56.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 100 mM NaCl

Signal Transduction

NP_002592

O43924

Human