Recombinant humaan PDE6H-eiwit

PDE6H is the inhibitory (or gamma) subunit of the cone-specific cGMP phosphodiesterase, which is a tetramer composed of two catalytic chains (alpha and beta), and two inhibitory chains (gamma) . It is specifically expressed in the retina, and is involved in the transmission and amplification of the visual signal. Mutations in this gene are associated with retinal cone dystrophy type 3A (RCD3A) . Recombinant human PDE6H protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Retinal cone rhodopsin-sensitive cGMP 3'5'-cyclic phosphodiesterase subunit gamma, Retinal cone rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma, ACHM6, RCD3

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

11.5 kDa (106aa) confirmed by MALDI-TOF

PDE6H, Retinal cone rhodopsin-sensitive cGMP 3'5'-cyclic phosphodiesterase subunit gamma, Retinal cone rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma, ACHM6, RCD3, ATGP2785-10 µg, ATGP2785-20 µg, ATGP2785-50 µg, ATGP2785-100 µg, ATGP2785-250 µg, ATGP2785-500 µg, ATGP2785-1 mg, ATGP2785-10, ATGP2785-20, ATGP2785-50, ATGP2785-100, ATGP2785-250, ATGP2785-500, ATGP2785-1

Shimizu-Matsumoto A., et al. (1996) Genomics 32:121-124.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 2mM DTT, 0.1mM PMSF

Signal Transduction

NP_006196

Q13956

Human

MSDNTTL PAPASNQGPT TPRKGPPKFK QRQTRQFKSK PPKKGVKGFG DDIPGMEGLG TDITVICPWE AFSHLELHEL AQFGII