Recombinant humaan PDGF R alfa-eiwit

PDGFRa, also known as platelet derived growth factor receptor alpha, is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. This protein can bind to both A and B subunits of PDGF. It is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes. Recombinant human PDGFRa protein, fused to His-tag at N-terminus, was expressed in E. coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Platelet-derived growth factor receptor alpha precursor, CD140A, PDGFR2, MGC74795, Rhe-PDGFRA

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

58.4 kDa (522aa)

PDGF R a, Platelet-derived growth factor receptor alpha precursor, CD140A, PDGFR2, MGC74795, Rhe-PDGFRA, ATGP1153-10 µg, ATGP1153-20 µg, ATGP1153-50 µg, ATGP1153-100 µg, ATGP1153-250 µg, ATGP1153-500 µg, ATGP1153-1 mg, ATGP1153-10, ATGP1153-20, ATGP1153-50, ATGP1153-100, ATGP1153-250, ATGP1153-500, ATGP1153-1

Yu J. et al. (2000) J Biol Chem. 275: 19076-82; ; Xie J. et al. (2001) . Proc. Natl. Acad. Sci. uSA 98: 9255-59

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Cancer

NP_006197

P16234

Human

MGSSHHHHHH SSGLVPRGSH MQLSLPSILP NENEKVVQLN SSFSLRCFGE SEVSWQYPMS EEESSDVEIR NEENNSGLFV TVLEVSSASA AHTGLYTCYY NHTQTEENEL EGRHIYIYVP DPDVAFVPLG MTDYLVIVED DDSAIIPCRT TDPETPVTLH NSEGVVPASY DSRQGFNGTF TVGPYICEAT VKGKKFQTIP FNVYALKATS ELDLEMEALK TVYKSGETIV VTCAVFNNEV VDLQWTYPGE VKGKGITMLE EIKVPSIKLV YTLTVPEATV KDSGDYECAA RQATREVKEM KKVTISVHEK GFIEIKPTFS QLEAVNLHEV KHFVVEVRAY PPPRISWLKN NLTLIENLTE ITTDVEKIQE IRYRSKLKLI RAKEEDSGHY TIVAQNEDAV KSYTFELLTQ VPSSILDLVD DHHGSTGGQT VRCTAEGTPL PDIEWMICKD IKKCNNETSW TILANNVSNI ITEIHSRDRS TVEGRVTFAK VEETIAVRCL AKNLLGAENR ELKLVAPTLR SE