Recombinant humaan PDIA4-eiwit

PDIA4, also known as ERP72, is an endoplasmic reticulum luminal protein that is both a stress protein and a member of the protein disulfide isomerase family of proteins. It is involved in the catalysis of protein-S-S-bond rearrangement. PDIA3 and PDIA4 act as proteases, protein disulfide isomerases, phospholipases or a combination of these. Recombinant human PDIA4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity > 5 A650/cm/min/mg. Enzymatic activity was confirmed by measuring the aggregation of insulin in the presence of DTT.

Protein disulfide-isomerase A4, Endoplasmic reticulum resident protein 72, ERP70, ERP72

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Specific activity > 5 A650/cm/min/mg. Enzymatic activity was confirmed by measuring the aggregation of insulin in the presence of DTT.

72.9 kDa (646aa) confirmed by MALDI-TOF

PDIA4, Protein disulfide-isomerase A4, Endoplasmic reticulum resident protein 72, ERP70, ERP72, ATGP3138-10 µg, ATGP3138-20 µg, ATGP3138-50 µg, ATGP3138-100 µg, ATGP3138-250 µg, ATGP3138-500 µg, ATGP3138-1 mg, ATGP3138-10, ATGP3138-20, ATGP3138-50, ATGP3138-100, ATGP3138-250, ATGP3138-500, ATGP3138-1

Thomas M., et al. (2010) Synapse. 64 (8) :579-93.; ; Joo JH., et al. (2007) Cancer Res. 67 (16) :7929-36.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl,1mM DTT and 10% glycerol

Heat Shock Proteins

NP_004902.1

P13667

Human

VAGAEGPDE DSSNRENAIE DEEEEEEEDD DEEEDDLEVK EENGVLVLND ANFDNFVADK DTVLLEFYAP WCGHCKQFAP EYEKIANILK DKDPPIPVAK IDATSASVLA SRFDVSGYPT IKILKKGQAV DYEGSRTQEE IVAKVREVSQ PDWTPPPEVT LVLTKENFDE VVNDADIILV EFYAPWCGHC KKLAPEYEKA AKELSKRSPP IPLAKVDATA ETDLAKRFDV SGYPTLKIFR KGRPYDYNGP REKYGIVDYM IEQSGPPSKE ILTLKQVQEF LKDGDDVIII GVFKGESDPA YQQYQDAANN LREDYKFHHT FSTEIAKFLK VSQGQLVVMQ PEKFQSKYEP RSHMMDVQGS TQDSAIKDFV LKYALPLVGH RKVSNDAKRY TRRPLVVVYY SVDFSFDYRA ATQFWRSKVL EVAKDFPEYT FAIADEEDYA GEVKDLGLSE SGEDVNAAIL DESGKKFAME PEEFDSDTLR EFVTAFKKGK LKPVIKSQPV PKNNKGPVKV VVGKTFDSIV MDPKKDVLIE FYAPWCGHCK QLEPVYNSLA KKYKGQKGLV IAKMDATAND VPSDRYKVEG FPTIYFAPSG DKKNPVKFEG GDRDLEHLSK FIEEHATKLS RTKEEL