Recombinant humaan PDLIM1-eiwit

PDLIM1 is a member of the enigma protein family. The protein contains two protein interacting domains, a PDZ domain at the amino terminal end and one to three LIM domains at the carboxyl terminal. It is a cytoplasmic protein associated with the cytoskeleton. The protein may function as an adapter to bring other LIM-interacting proteins to the cytoskeleton. Pseudogenes associated with this gene are located on chromosomes 3, 14 and 17. Recombinant human PDLIM1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

PDZ and LIM domain protein 1, CLIM1, CLP-36, CLP36, Hclim1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

38.7 kDa (354aa) confirmed by MALDI-TOF

PDLIM1, PDZ and LIM domain protein 1, CLIM1, CLP-36, CLP36, Hclim1, ATGP2527-10 µg, ATGP2527-20 µg, ATGP2527-50 µg, ATGP2527-100 µg, ATGP2527-250 µg, ATGP2527-500 µg, ATGP2527-1 mg, ATGP2527-10, ATGP2527-20, ATGP2527-50, ATGP2527-100, ATGP2527-250, ATGP2527-500, ATGP2527-1

Bauer K., et al. (2000) Blood. 96: 4236-4245.; ; Vallenius T., et al. (2002) J Cell Sci. 115: 2067-2073.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 0.1M NaCl, 10% glycerol, 1mM DTT, 2mM EDTA

Metabolism

NP_066272

O00151

Human

MTTQQ IDLQGPGPWG FRLVGGKDFE QPLAISRVTP GSKAALANLC IGDVITAIDG ENTSNMTHLE AQNRIKGCTD NLTLTVARSE HKVWSPLVTE EGKRHPYKMN LASEPQEVLH IGSAHNRSAM PFTASPASST TARVITNQYN NPAGLYSSEN ISNFNNALES KTAASGVEAN SRPLDHAQPP SSLVIDKESE VYKMLQEKQE LNEPPKQSTS FLVLQEILES EEKGDPNKPS GFRSVKAPVT KVAASIGNAQ KLPMCDKCGT GIVGVFVKLR DRHRHPECYV CTDCGTNLKQ KGHFFVEDQI YCEKHARERV TPPEGYEVVT VFPK