Recombinant humaan PDXP-eiwit

Pyridoxal phosphate phosphatase, also known as PDXP is the enzyme belongs to the family of hydrolases, specifically those acting on phosphoric monoester bonds. PDXP catalyzes the dephosphorylation of pyridoxal 5'-phosphate and exhibits a high level of expression various parts of the central nervous system, especially the brain. PDXP activity is catalyzed by haloacid dehalogenase (HAD), and it is the cofactor of both aromatic L-amino acid decarboxylase and glutamate decarboxylase. Recombinant human PDXP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Pyridoxal phosphate phosphatase, CIN, PLP, PLPP, Pyridoxal phosphate phosphatase

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

33.8 kDa (316aa) confirmed by MALDI-TOF

PDXP, Pyridoxal phosphate phosphatase, CIN, PLP, PLPP, Pyridoxal phosphate phosphatase, ATGP0652-10 µg, ATGP0652-20 µg, ATGP0652-50 µg, ATGP0652-100 µg, ATGP0652-250 µg, ATGP0652-500 µg, ATGP0652-1 mg, ATGP0652-10, ATGP0652-20, ATGP0652-50, ATGP0652-100, ATGP0652-250, ATGP0652-500, ATGP0652-1

Jang YM., et al. (1992) J Biol Chem. 278 (50) :50040-6.; ; Fonda ML., et al. (1992) J Biol Chem. 267 (22) :15978-83.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 0.1M NaCl

Metabolism

NP_064711

Q96GD0

Human

MGSSHHHHHH SSGLVPRGSH MARCERLRGA ALRDVLGRAQ GVLFDCDGVL WNGERAVPGA PELLERLARA GKAALFVSNN SRRARPELAL RFARLGFGGL RAEQLFSSAL CAARLLRQRL PGPPDAPGAV FVLGGEGLRA ELRAAGLRLA GDPSAGDGAA PRVRAVLVGY DEHFSFAKLR EACAHLRDPE CLLVATDRDP WHPLSDGSRT PGTGSLAAAV ETASGRQALV VGKPSPYMFE CITENFSIDP ARTLMVGDRL ETDILFGHRC GMTTVLTLTG VSRLEEAQAY LAAGQHDLVP HYYVESIADL TEGLED