Recombinant humaan PECI/ECI2-eiwit

PECI, also referred to as DRS1, ACBD2 or HCA88, is a protein that localizes to the peroxisomal matrix and contains one ACB (acyl-CoA-binding) domain. This protein is expressed abundantly in liver, heart and skeletal muscle. It functions to catalyze the isomerization of both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species, playing an important role in the beta-oxidation of unsaturated fatty acids. Recombinant human PECI protein, fused to his-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Enoyl-CoA delta isomerase 2, D3, D2-enoyl-CoA isomerase, Diazepam-binding inhibitor-related protein 1, DBI-related protein 1, Dodecenoyl-CoA isomerase, Hepatocellular carcinoma-associated antigen 88, Peroxisomal 3,2-trans-enoyl-CoA isomerase, Renal carcinoma antigen NY-REN-1, ACBD2, DRS1, HCA88

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

42.3 kDa (384aa) confirmed by MALDI-TOF

PECI, Peroxisomal 32-trans-enoyl-CoA isomerase, ACBD2, DRS1, HCA88, Peroxisomal 3,2-trans-enoyl-CoA isomerase, acyl Coenzyme A binding domain containing 2, D3 D2 enoyl CoA isomerase, DBI related protein 1, Delta (3) delta (2) enoyl CoA isomerase, Dodecenoyl CoA isomerase, DRS 1, HCA 88, Hepatocellular carcinoma associated antigen 88, Peroxisomal D3 D2 enoyl CoA isomerase, Renal carcinoma antigen NY REN 1, ATGP0605-10 µg, ATGP0605-20 µg, ATGP0605-50 µg, ATGP0605-100 µg, ATGP0605-250 µg, ATGP0605-500 µg, ATGP0605-1 mg, ATGP0605-10, ATGP0605-20, ATGP0605-50, ATGP0605-100, ATGP0605-250, ATGP0605-500, ATGP0605-1

Dongyan Zhang, et al. (2002) J. Biol. Chem. 277: 9127-9132.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Enzymes & Proteases

NP_006108

O75521

Human

MNRTAMRASQ KDFENSMNQV KLLKKDPGNE VKLKLYALYK QATEGPCNMP KPGVFDLINK AKWDAWNALG SLPKEAARQN YVDLVSSLSP SLESSSQVEP GTDRKSTGFE TLVVTSEDGI TKIMFNRPKK KNAINTEMYH EIMRALKAAS KDDSIITVLT GNGDYYSSGN DLTNFTDIPP GGVEEKAKNN AVLLREFVGC FIDFPKPLIA VVNGPAVGIS VTLLGLFDAV YASDRATFHT PFSHLGQSPE GCSSYTFPKI MSPAKATEML IFGKKLTAGE ACAQGLVTEV FPDSTFQKEV WTRLKAFAKL PPNALRISKE VIRKREREKL HAVNAEECNV LQGRWLSDEC TNAVVNFLSR KSKL