Recombinant humaan PECR-eiwit

Peroxisomal trans-2-enoyl-CoA reductase, also known as PECR, the key enzyme for a proposed peroxisomal chain elongation pathway. This proein was mainly expressed in liver and kidney. Recombinant human PECR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Peroxisomal trans-2-enoyl-CoA reductase, DCRRP, HPDHASE, HSA250303, PVIARL, SDR29C1, TERP

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

35.1 kDa (327aa) confirmed by MALDI-TOF

PECR, Peroxisomal trans-2-enoyl-CoA reductase, DCRRP, HPDHASE, HSA250303, PVIARL, SDR29C1, TERP, ATGP1239-10 µg, ATGP1239-20 µg, ATGP1239-50 µg, ATGP1239-100 µg, ATGP1239-250 µg, ATGP1239-500 µg, ATGP1239-1 mg, ATGP1239-10, ATGP1239-20, ATGP1239-50, ATGP1239-100, ATGP1239-250, ATGP1239-500, ATGP1239-1

Das AK., et al. (2000) J Biol Chem. 275 (32) :24333-40.; ; Amery L., et al. (2001) Comb Chem High Throughput Screen. 4 (7) :545-52.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.15M NaCl.

Metabolism

NP_060911

Q9BY49

Human

MGSSHHHHHH SSGLVPRGSH MGSHMASWAK GRSYLAPGLL QGQVAIVTGG ATGIGKAIVK ELLELGSNVV IASRKLERLK SAADELQANL PPTKQARVIP IQCNIRNEEE VNNLVKSTLD TFGKINFLVN NGGGQFLSPA EHISSKGWHA VLETNLTGTF YMCKAVYSSW MKEHGGSIVN IIVPTKAGFP LAVHSGAARA GVYNLTKSLA LEWACSGIRI NCVAPGVIYS QTAVENYGSW GQSFFEGSFQ KIPAKRIGVP EEVSSVVCFL LSPAASFITG QSVDVDGGRS LYTHSYEVPD HDNWPKGAGD LSVVKKMKET FKEKAKL