Recombinant humaan PEDF/SERPINF1-eiwit

PEDF, also known as SERPINF1, is an extracellular neurotrophic agent, first identified as a secreted product in conditioned medium from cultured fetal human retinal pigment epithelial (RPE) cells. It was reported that PEDF isolated from medium conditioned by human fetal RPE primary cultures promotes neurite outgrowth in cultured human retinoblastoma Y-79 cells. PEDF is also a member of the serpin family, a group of serine protease inhibitors. As PEDF does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. Recombinant PEDF protein containing a natural variant T72M was expressed in E. coli and purified by using conventional chromatography techniques.

Pigment epithelium-derived factor, Pigment epithelium-derived factor, EPC-1, PIG35, SERPINF1, Pigment epithelium-derived factor EPC 1, EPC1, PIG 35, Pigment epithelium derived factor, Proliferation inducing protein 35, SERPIN F1, Serpin peptidase inhibitor clade F member 1, SERPINF 1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

46.7 kDa (420aa) confirmed by MALDI-TOF

SERPIN F1, PEDF, Pigment epithelium-derived factor, Pigment epithelium-derived factor, EPC-1, PIG35, SERPINF1, Pigment epithelium-derived factor EPC 1, EPC1, PIG 35, Pigment epithelium derived factor, Proliferation inducing protein 35, SERPIN F1, Serpin peptidase inhibitor clade F member 1, SERPINF 1, ATGP0342-10 µg, ATGP0342-20 µg, ATGP0342-50 µg, ATGP0342-100 µg, ATGP0342-250 µg, ATGP0342-500 µg, ATGP0342-1 mg, ATGP0342-10, ATGP0342-20, ATGP0342-50, ATGP0342-100, ATGP0342-250, ATGP0342-500, ATGP0342-1

Steele FR., et al. (1993) Proc Natl Acad Sci u S A. 90 (4) :1526-30..; ; Becerra SP., et al. (1995) J Biol Chem. 270 (43) :25992-9.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol

Angiogenesis

NP_002606.3

P36955

Human

MGSSHHHHHH SSGLVPRGSH MQNPASPPEE GSPDPDSTGA LVEEEDPFFK VPVNKLAAAV SNFGYDLYRV RSSMSPTTNV LLSPLSVATA LSALSLGAEQ RTESIIHRAL YYDLISSPDI HGTYKELLDT VTAPQKNLKS ASRIVFEKKL RIKSSFVAPL EKSYGTRPRV LTGNPRLDLQ EINNWVQAQM KGKLARSTKE IPDEISILLL GVAHFKGQWV TKFDSRKTSL EDFYLDEERT VRVPMMSDPK AVLRYGLDSD LSCKIAQLPL TGSMSIIFFL PLKVTQNLTL IEESLTSEFI HDIDRELKTV QAVLTVPKLK LSYEGEVTKS LQEMKLQSLF DSPDFSKITG KPIKLTQVEH RAGFEWNEDG AGTTPSPGLQ PAHLTFPLDY HLNQPFIFVL RDTDTGALLF IGKILDPRGP